文献类型： 外文期刊
作者： Chen, Yibao 1 ; Yan, Bingjie 1 ; Chen, Weizhong 3 ; Zhang, Xue 1 ; Liu, Zhengjie 1 ; Zhang, Qing 1 ; Li, Lulu 1 ; Hu, Ming 1 ; Zhao, Xiaonan 1 ; Xu, Xiaohui 1 ; Lv, Qianghua 1 ; Luo, Yanbo 1 ; Cai, Yumei 4 ; Liu, Yuqing 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan, Peoples R China
2.China UK Joint Lab Bacteriophage Engn, Jinan, Peoples R China
3.Ningbo Univ, Sch Marine Sci, Ningbo, Peoples R China
4.Shandong Agr Univ, Coll Vet Med, Tai An, Peoples R China
5.Shandong Vamph Anim Hlth Prod Co LTD, Jinan, Peoples R China
期刊名称：ISCIENCE （ 影响因子：4.6； 五年影响因子：5.0 ）
ISSN：
年卷期： 2024 年 27 卷 7 期
页码：
收录情况： SCI
摘要： Pseudomonas aeruginosa is a common opportunistic pathogen. The potential efficacy of phage therapy has attracted the attention of researchers, but efficient gene-editing tools are lacking, limiting the study of their biological properties. Here, we designed a type V CRISPR-Cas12a system for the gene editing of P. aeruginosa phages. We first evaluated the active cutting function of the CRISPR-Cas12a system in vitro and discovered that it had a higher gene-cutting efficiency than the type II CRISPR-Cas9 system in three different P. aeruginosa phages. We also demonstrated the system's ability to precisely edit genes in Escherichia coli phages, Salmonella phages, and P. aeruginosa phages. Using the aforementioned strategies, non-essential P. aeruginosa phage genes can be efficiently deleted, resulting in a reduction of up to 5,215 bp (7.05%). Our study has provided a rapid, efficient, and time-saving tool that accelerates progress in phage engineering.
- 相关文献
作者其他论文 更多>>
-
Recombinase polymerase amplification combined with lateral flow biosensor for rapid visual detection of Clostridium perfringens in chicken meat and milk
作者：Tian, Rui;Xie, Feng;Liu, Guangjin;Zhang, Wei;Tian, Rui;Xie, Feng;Liu, Guangjin;Zhang, Wei;Liu, Yuqing;Li, Qingxia;Wang, Jinxiu;Dai, Lei;Zhang, Hongjian
关键词：foodborne pathogen; Clostridium perfringens; on-site detection method; recombinase polymerase amplification; lateral flow biosensor
-
Salmonella enteritidis acquires phage resistance through a point mutation in rfbD but loses some of its environmental adaptability
作者：Zeng, Yukun;Li, Ping;Liu, Shenglong;Shen, Mangmang;Zhou, Xin;Zeng, Yukun;Zhou, Xin;Zeng, Yukun;Zhou, Xin;Liu, Yuqing
关键词：Salmonella enteritidis; rfbD gene; point mutation; phage resistance; environmental adaptability
-
Antimicrobial Resistance and Molecular Characterization of Escherichia coli from Healthy Chickens in Shandong, China from 2009 to 2014
作者：Li, Lulu;Zhao, Xiaonan;Zhang, Qing;Luo, Yanbo;Hu, Ming;Chen, Yibao;Liu, Yuqing;Li, Lulu;Zhao, Xiaonan;Zhang, Qing;Luo, Yanbo;Hu, Ming;Chen, Yibao;Liu, Yuqing;Guo, Genglin
关键词：Escherichia coli; antimicrobial resistance; ESBL; PMQR; PMCR
-
Toxin gene detection and antibiotic resistance of Clostridium perfringens from aquatic sources
作者：Li, Mengxuan;Wang, Ying;Hou, Bingyu;Cai, Yumei;Chen, Yibao;Hu, Ming;Zhao, Xiaonan;Zhang, Qing;Li, Lulu;Luo, Yanbo;Liu, Yuqing
关键词：Aquatic animals; Clostridium perfringens; Toxin gene; Antibiotic resistance phenotype; Clam
-
Efficacy of a new K3-specific bacteriophage for controlling Klebsiella pneumoniae in milk and its potential to disrupt biofilm formation
作者：Li, Pei;Zhou, Yu;Qin, Xiayan;Hu, Zimeng;Ma, Jiale;Zhang, Wei;Li, Pei;Zhou, Yu;Hu, Zimeng;Zhang, Wei;Guo, Genglin;Li, Min;Tan, Zhongming;Liu, Yuqing;Han, Xiangan;Du, Hong;Zhang, Wei
关键词：Klebsiella pneumoniae; Milk; Bacteriophage; K3; Biofilm; Depolymerase
-
Genomic analysis and experimental pathogenic characterization of Riemerella anatipestifer isolates from chickens in China
作者：Chen, Yibao;Liu, Zhengjie;Hu, Ming;Luo, Yanbo;Zhang, Qing;Li, Lulu;Zhao, Xiaonan;Zhao, Min;Liu, Yuqing;Li, Xiaojing;Luo, Yanbo;Liu, Wenhua;Ma, Jinxiang
关键词：R. anatipestifer; genome analysis; infection chicken; jelly-like; vertically
-
Identification of the mutual gliding locus as a factor for gut colonization in non-native bee hosts using the ARTP mutagenesis
作者：Meng, Yujie;Zheng, Hao;Meng, Yujie;Li, Yuan;Shao, Zenghua;Liu, Shanshan;Zhang, Xue;Zhai, Yifan;Zhang, Chong;Xing, Xin-Hui
关键词：ARTP; Mutagenesis; Gut microbiota; MglB; Host colonization; Bumblebee; Honeybee
