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Identification of the mutual gliding locus as a factor for gut colonization in non-native bee hosts using the ARTP mutagenesis

文献类型： 外文期刊

作者： Meng, Yujie 1 ; Zhang, Xue 3 ; Zhai, Yifan 4 ; Li, Yuan 2 ; Shao, Zenghua 2 ; Liu, Shanshan 2 ; Zhang, Chong 5 ; Xing, Xin-Hui 6 ; Zheng, Hao 1 ;

作者机构： 1.Kunming Univ Sci & Technol, Fac Food Sci & Engn, Kunming 650500, Peoples R China

2.MGI Tech, Qingdao 266426, Peoples R China

3.China Agr Univ, Coll Plant Protect, Dept Entomol, Beijing 100083, Peoples R China

4.Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Peoples R China

5.Tsinghua Univ, Inst Biochem Engn, Dept Chem Engn, Beijing 100084, Peoples R China

6.Tsinghua Univ, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China

关键词： ARTP; Mutagenesis; Gut microbiota; MglB; Host colonization; Bumblebee; Honeybee

期刊名称：MICROBIOME （ 影响因子：15.5； 五年影响因子：19.4 ）

ISSN： 2049-2618

年卷期： 2024 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Background The gut microbiota and their hosts profoundly affect each other's physiology and evolution. Identifying host-selected traits is crucial to understanding the processes that govern the evolving interactions between animals and symbiotic microbes. Current experimental approaches mainly focus on the model bacteria, like hypermutating Escherichia coli or the evolutionary changes of wild stains by host transmissions. A method called atmospheric and room temperature plasma (ARTP) may overcome the bottleneck of low spontaneous mutation rates while maintaining mild conditions for the gut bacteria.Results We established an experimental symbiotic system with gnotobiotic bee models to unravel the molecular mechanisms promoting host colonization. By in vivo serial passage, we tracked the genetic changes of ARTP-treated Snodgrassella strains from Bombus terrestris in the non-native honeybee host. We observed that passaged isolates showing genetic changes in the mutual gliding locus have a competitive advantage in the non-native host. Specifically, alleles in the orphan mglB, the GTPase activating protein, promoted colonization potentially by altering the type IV pili-dependent motility of the cells. Finally, competition assays confirmed that the mutations out-competed the ancestral strain in the non-native honeybee gut but not in the native host.Conclusions Using the ARTP mutagenesis to generate a mutation library of gut symbionts, we explored the potential genetic mechanisms for improved gut colonization in non-native hosts. Our findings demonstrate the implication of the cell mutual-gliding motility in host association and provide an experimental system for future study on host-microbe interactions. ArN8Xjm3N6Dubzus4DCSQm Video AbstractConclusions Using the ARTP mutagenesis to generate a mutation library of gut symbionts, we explored the potential genetic mechanisms for improved gut colonization in non-native hosts. Our findings demonstrate the implication of the cell mutual-gliding motility in host association and provide an experimental system for future study on host-microbe interactions. ArN8Xjm3N6Dubzus4DCSQm Video Abstract

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