Toxin gene detection and antibiotic resistance of Clostridium perfringens from aquatic sources
文献类型： 外文期刊
作者： Li, Mengxuan 1 ; Wang, Ying 1 ; Hou, Bingyu 1 ; Chen, Yibao 2 ; Hu, Ming 2 ; Zhao, Xiaonan 2 ; Zhang, Qing 2 ; Li, Lulu 2 ; Luo, Yanbo 2 ; Liu, Yuqing 2 ; Cai, Yumei 1 ;
作者机构： 1.Shandong Agr Univ, Coll Vet Med, Tai An, Peoples R China
2.Shandong Acad Agr Sci, Jinan, Peoples R China
关键词： Aquatic animals; Clostridium perfringens; Toxin gene; Antibiotic resistance phenotype; Clam
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.4； 五年影响因子：5.5 ）
ISSN： 0168-1605
年卷期： 2024 年 415 卷
页码：
收录情况： SCI
摘要： Clostridium perfringens is a zoonotic opportunistic pathogen that produces toxins that can cause necrotic enteritis and even "sudden death disease". This bacterium is widely distributed in the intestines of livestock and human, but there are few reports of distribution in aquatic animals (Hafeez et al., 2022). In order to explore the isolation rate of C. perfringens and the toxin genes they carry, 141 aquatic samples, including clams (Ruditapes philippinarum), oysters (Ostreidae), and mud snails (Bullacta exerata Philippi), were collected from the coastal areas of Shandong Province, China. C. perfringens strains were tested for cpa, cpb, etx, iap, cpb2, cpe, netB, and tpeL genes. 45 clam samples were boiled at 100 degrees C for 5 min before bacteria isolation. 80 strains were isolated from 141 samples with the positive rate being 57 %.And the positive rates of cooked clams was 87 % which was higher than the average. In detection of 8 toxin genes, all strains tested cpa positive, 3 strains netB positive, and 2 cpb and cpe, respectively. 64 strains were selected to analyze the antibiotic resistance phenotype of 10 antibiotics. The average antibiotic resistance rates of the strains to tetracycline, clindamycin, and ampicillin were 45 %, 20 %, and 16 % respectively, and the MIC of 4 strains to clindamycin was >= 128 mu g/mL. A high isolation rate of C. perfringens from aquatic animals was shown, and it was isolated from boiled clams for the first time, in which cpe and netB toxin genes were detected for the first time too. The toxin encoded by cpe gene can cause food poisoning of human, thus the discoveries of this study have certain guiding significance for food safety. Antibiotics resistant C. perfringens of aquatic origin may arise from transmission in the terrestrial environment or from antibiotic contamination of the aquaculture environment and is of public health significance.
- 相关文献
作者其他论文 更多>>
-
Recombinase polymerase amplification combined with lateral flow biosensor for rapid visual detection of Clostridium perfringens in chicken meat and milk
作者：Tian, Rui;Xie, Feng;Liu, Guangjin;Zhang, Wei;Tian, Rui;Xie, Feng;Liu, Guangjin;Zhang, Wei;Liu, Yuqing;Li, Qingxia;Wang, Jinxiu;Dai, Lei;Zhang, Hongjian
关键词：foodborne pathogen; Clostridium perfringens; on-site detection method; recombinase polymerase amplification; lateral flow biosensor
-
Salmonella enteritidis acquires phage resistance through a point mutation in rfbD but loses some of its environmental adaptability
作者：Zeng, Yukun;Li, Ping;Liu, Shenglong;Shen, Mangmang;Zhou, Xin;Zeng, Yukun;Zhou, Xin;Zeng, Yukun;Zhou, Xin;Liu, Yuqing
关键词：Salmonella enteritidis; rfbD gene; point mutation; phage resistance; environmental adaptability
-
Antimicrobial Resistance and Molecular Characterization of Escherichia coli from Healthy Chickens in Shandong, China from 2009 to 2014
作者：Li, Lulu;Zhao, Xiaonan;Zhang, Qing;Luo, Yanbo;Hu, Ming;Chen, Yibao;Liu, Yuqing;Li, Lulu;Zhao, Xiaonan;Zhang, Qing;Luo, Yanbo;Hu, Ming;Chen, Yibao;Liu, Yuqing;Guo, Genglin
关键词：Escherichia coli; antimicrobial resistance; ESBL; PMQR; PMCR
-
Efficacy of a new K3-specific bacteriophage for controlling Klebsiella pneumoniae in milk and its potential to disrupt biofilm formation
作者：Li, Pei;Zhou, Yu;Qin, Xiayan;Hu, Zimeng;Ma, Jiale;Zhang, Wei;Li, Pei;Zhou, Yu;Hu, Zimeng;Zhang, Wei;Guo, Genglin;Li, Min;Tan, Zhongming;Liu, Yuqing;Han, Xiangan;Du, Hong;Zhang, Wei
关键词：Klebsiella pneumoniae; Milk; Bacteriophage; K3; Biofilm; Depolymerase
-
The W195 Residue of the Newcastle Disease Virus V Protein Is Critical for Multiple Aspects of Viral Self-Regulation through Interactions between V and Nucleoproteins
作者：Wei, Qiaolin;Meng, Fanxing;Wang, Ying;Wei, Ning;Tian, Jianxia;Li, Hanlue;Hao, Qiqi;Zhou, Zijie;Liu, Haijin;Yang, Zengqi;Xiao, Sa;Wang, Wenbin
关键词：Newcastle disease virus; V protein; minigenome; inclusion body formation
-
Genomic analysis and experimental pathogenic characterization of Riemerella anatipestifer isolates from chickens in China
作者：Chen, Yibao;Liu, Zhengjie;Hu, Ming;Luo, Yanbo;Zhang, Qing;Li, Lulu;Zhao, Xiaonan;Zhao, Min;Liu, Yuqing;Li, Xiaojing;Luo, Yanbo;Liu, Wenhua;Ma, Jinxiang
关键词：R. anatipestifer; genome analysis; infection chicken; jelly-like; vertically
-
Development of the CRISPR-Cas12a system for editing of Pseudomonas aeruginosa phages
作者：Chen, Yibao;Yan, Bingjie;Zhang, Xue;Liu, Zhengjie;Zhang, Qing;Li, Lulu;Hu, Ming;Zhao, Xiaonan;Xu, Xiaohui;Lv, Qianghua;Luo, Yanbo;Liu, Yuqing;Chen, Yibao;Yan, Bingjie;Zhang, Xue;Liu, Zhengjie;Zhang, Qing;Li, Lulu;Hu, Ming;Zhao, Xiaonan;Xu, Xiaohui;Lv, Qianghua;Luo, Yanbo;Liu, Yuqing;Chen, Weizhong;Cai, Yumei;Chen, Yibao;Hu, Ming;Liu, Yuqing
关键词：
