Efficacy of a new K3-specific bacteriophage for controlling Klebsiella pneumoniae in milk and its potential to disrupt biofilm formation
文献类型： 外文期刊
作者： Li, Pei 1 ; Zhou, Yu 1 ; Guo, Genglin 3 ; Qin, Xiayan 1 ; Hu, Zimeng 1 ; Li, Min 4 ; Tan, Zhongming 5 ; Liu, Yuqing 6 ; Han, Xiangan 7 ; Ma, Jiale 1 ; Du, Hong 8 ; Zhang, Wei 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Key Lab Anim Bacteriol,Minist Agr, Nanjing 210095, Peoples R China
2.Nanjing Agr Univ, Sanya Inst, Yabulun Ind Pk,Yazhou Bay Sci & Technol City, Sanya 572024, Peoples R China
3.Shandong Acad Agr Sci, Shandong Inst Sericulture, Yantai, Peoples R China
4.Jiangsu Univ, Sch Med, Dept Biochem & Mol Biol, Zhenjiang 212013, Jiangsu, Peoples R China
5.Jiangsu Prov Ctr Dis Control & Prevent, NHC Key Lab Enter Pathogen Microbiol, Nanjing 210014, Peoples R China
6.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Shandong, Peoples R China
7.Chinese Acad Agr Sci CAAS, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China
8.Soochow Univ, Dept Clin Lab, Affiliated Hosp 2, Suzhou 215004, Peoples R China
9.Xinjiang Agr Univ, Coll Vet Med, Urumqi 830052, Peoples R China
关键词： Klebsiella pneumoniae; Milk; Bacteriophage; K3; Biofilm; Depolymerase
期刊名称：FOOD CONTROL （ 影响因子：6.0； 五年影响因子：5.8 ）
ISSN： 0956-7135
年卷期： 2024 年 163 卷
页码：
收录情况： SCI
摘要： Klebsiella pneumoniae ( K. pneumoniae ) has caused huge economic loss in the dairy industry by inducing mastitis and even endanger public health through contaminated milk products. Suppressing bacterial proliferation in raw milk, particularly in cold settings, is important to preserve milk quality. Owing to increasingly serious antibiotic resistance, bacterial infection has gradually become a difficult matter to settle. Thus, bacteriophages emerge as a potentially effective substitute for antibiotics, garnering renewed interest. Here, we isolated and characterized a new phage, designated P1010, which lyses K3 K. pneumoniae specifically among 23 serotypes. The phage exhibited a latent time of 8 min and produced roughly 101 phages per infected bacteria. Possessing a genome of 44,270 base pairs, the linear double-stranded DNA molecule was devoid of lysogeny, toxin, or tRNA-relevant genes. Of note, P1010 succeeded in significantly suppress bacterial growth in milk both under cold storage condition and room temperature. During the biofilm formation inhibition experiment, OD 595 values and viable bacteria counts in the phage treatment groups were significantly reduced. A depolymerase derived from P1010 was identified, possessing a K3 specific enzymatic spectrum. In summary, Klebsiella phage P1010 exhibited bright prospects as biocontrol agents in controlling K. pneumoniae contamination in the dairy industry, offering a viable alternative to antibiotics.
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