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Membralin is required for maize development and defines a branch of the endoplasmic reticulum-associated degradation pathway in plants

文献类型： 外文期刊

作者： Liu, Baiyu 1 ; Xu, Changzheng 2 ; He, Qiuxia 3 ; Zhang, Ke 1 ; Qi, Shoumei 1 ; Jin, Zhe 1 ; Cheng, Wen 4 ; Ding, Zhaohua 4 ; Chen, Donghua 1 ; Zhao, Xiangyu 5 ; Zhang, Wei 1 ; Zhang, Kewei 1 ; Li, Kunpeng 1 ;

作者机构： 1.Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Peoples R China

2.Southwest Univ, Sch Life Sci, Chongqing 400715, Peoples R China

3.Qilu Univ Technol, Shandong Acad Sci, Sci & Technol Serv Platform, Jinan 250103, Peoples R China

4.Shandong Acad Agr Sci, Maize Inst, Jinan 250100, Shandong, Peoples R China

5.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

关键词： endoplasmic reticulum-associated degradation; maize; membralin; plant development; unfold protein response

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：9.4； 五年影响因子：10.8 ）

ISSN： 0027-8424

年卷期： 2024 年 121 卷 25 期

页码：

收录情况： SCI

摘要： Endoplasmic reticulum (ER)-associated degradation (ERAD) plays key roles in controlling protein levels and quality in eukaryotes. The Ring Finger Protein 185 (RNF185)/ membralin ubiquitin ligase complex was recently identified as a branch in mammals and is essential for neuronal function, but its function in plant development is unknown. Here, we report the map- based cloning and characterization of Narrow Leaf and Dwarfism 1 (NLD1), which encodes the ER membrane-localized protein membralin and specifically interacts with maize homologs of RNF185 and related components. The nld1 mutant shows defective leaf and root development due to reduced cell number. The defects of nld1 were largely restored by expressing membralin genes from Arabidopsis thaliana and mice, highlighting the conserved roles of membralin proteins in animals and plants. The excessive accumulation of (3- hydroxy (3- methylglutaryl- CoA reductase in nld1 indicates that the enzyme is a membralin- mediated ERAD target. The activation of bZIP60 mRNA splicing-related unfolded protein response signaling and marker gene expression in nld1, as well as DNA fragment and cell viability assays, indicate that membralin deficiency induces ER stress and cell death in maize, thereby affecting organogenesis. Our findings uncover the conserved, indispensable role of the membralin- mediated branch of the ERAD pathway in plants. In addition, ZmNLD1 contributes to plant architecture in a dose- dependent manner, which can serve as a potential target for genetic engineering to shape ideal plant architecture, thereby enhancing high- density maize yields.

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