Sifting through the core-genome to identify putative cross-protective antigens against Riemerella anatipestifer
文献类型： 外文期刊
作者： Zheng, Xiangkuan 1 ; Xu, Sixiang 1 ; Wang, Zhuohao 1 ; Tao, Xingyu 1 ; Liu, Yuqing 5 ; Dai, Lei 6 ; Li, Yubao 7 ; Zhang, Wei 1 ;
作者机构： 1.Sanya Inst Nanjing Agr Univ, Sanya 572024, Peoples R China
2.Minist Agr, Key Lab Anim Bacteriol, Nanjing 210095, Peoples R China
3.OIE Reference Lab Swine Streptococcosis, Nanjing 210095, Peoples R China
4.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
5.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Shandong, Peoples R China
6.Hainan Anim Dis Prevent & Control Ctr, 16 Xingdan Rd, Haikou 571100, Peoples R China
7.Liaocheng Univ, Agr Sci & Engn Sch, Liaocheng, Peoples R China
关键词： Riemerella anatipestifer; Core-genome; Pan-genome; Reverse vaccinology; Cross-protective antigens; Subunit vaccine
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：5.0； 五年影响因子：5.2 ）
ISSN： 0175-7598
年卷期： 2023 年 107 卷 9 期
页码：
收录情况： SCI
摘要： Infectious serositis of ducks, caused by Riemerella anatipestifer, is one of the main infectious diseases that harm commercial ducks. Whole-strain-based vaccines with no or few cross-protection were observed between different serotypes of R. anatipestifer, and so far, control of infection is hampered by a lack of effective vaccines, especially subunit vaccines with cross-protection. Since the concept of reverse vaccinology was introduced, it has been widely used to screen for protective antigens in important pathogens. In this study, pan-genome binding reverse vaccinology, an emerging approach to vaccine candidate screening, was used to screen for cross-protective antigens against R. anatipestifer. Thirty proteins were identified from the core-genome as potential cross-protective antigens. Three of these proteins were recombinantly expressed, and their immunoreactivity with five antisera (anti-serotypes 1, 2, 6, 10, and 11) was demonstrated by Western blotting. Our study established a method for high-throughput screening of cross-protective antigens against R. anatipestifer in silico, which will lay the foundation for the development of a cross-protective subunit vaccine controlling R. anatipestifer infection.
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