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Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

文献类型： 外文期刊

作者： Hu, Ming 1 ; Liu, Zhengjie 1 ; Song, Ziang 1 ; Li, Lulu 1 ; Zhao, Xiaonan 1 ; Luo, Yanbo 1 ; Zhang, Qing 1 ; Chen, Yibao 1 ; Xu, Xiaohui 1 ; Dong, Yahui 1 ; Hrabchenko, Nataliia 1 ; Zhang, Wei 3 ; Liu, Yuqing 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Key Lab Livestock & Poultry Multi MARA, Jinan 251000, Peoples R China

2.Univ Leicester, Dept Genet & Genomes Biol, Leicester, England

3.Nanjing Agr Univ, Coll Vet Med, Nanjing, Peoples R China

期刊名称：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS （ 影响因子：1.2； 五年影响因子：1.6 ）

ISSN： 1940-087X

年卷期： 2023 年 197 期

页码：

收录情况： SCI

摘要： To improve the efficiency of antimicrobial susceptibility testing (AST) and phage high-throughput screening for resistant bacteria and to reduce the detection cost, an intelligent high-throughput AST/phage screening system, including a 96-dot matrix inoculator, image acquisition converter, and corresponding software, was developed according to AST criteria and the breakpoints of resistance (R) formulated by the Clinical & Laboratory Standards Institute (CLSI). AST and statistics of minimum inhibitory concentration (MIC) distributions (from R/8 to 8R) of 1,500 Salmonella strains isolated from poultry in Shandong, China, against 10 antimicrobial agents were carried out by the intelligent high-throughput AST/phage screening system. The Lar index, meaning "less antibiosis, less resistance and residual until little antibiosis", was obtained by calculating the weighted average of each MIC and dividing by R. This approach improves accuracy in comparison with using the prevalence of resistance to characterize the antimicrobial resistance (AMR) degree of highly resistant strains. For the strains of Salmonella with high AMR, lytic phages were efficiently screened from the phage library by this system, and the lysis spectrum was computed and analyzed. The results showed that the intelligent high-throughput AST/phage screening system was operable, accurate, highly efficient, inexpensive, and easy to maintain. Combined with the Shandong veterinary antimicrobial resistance monitoring system, the system was suitable for scientific research and clinical detection related to AMR.

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