Identification and Molecular Modification of Staphylococcus aureus Bacteriophage Lysin LysDZ25
文献类型： 外文期刊
作者： Chang, Yan 1 ; Li, Qingbin 1 ; Zhang, Shuhang 1 ; Zhang, Qing 1 ; Qi, Qingsheng 2 ; Lu, Xuemei 1 ; Liu, Yuqing 1 ;
作者机构： 1.Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China
2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China
关键词： LysDZ25; thermal stability; lytic activity; MD simulation; MDL method
期刊名称：ACS INFECTIOUS DISEASES （ 影响因子：5.3； 五年影响因子：5.1 ）
ISSN： 2373-8227
年卷期： 2023 年
页码：
收录情况： SCI
摘要： With the continuous emergence and spread of drug resistant and multi-drug-resistant Staphylococcus aureus, traditional antibiotic treatment has gradually lost its effect. There is an urgent need to develop and study new and effective bio-green inhibitors to control S. aureus. In this study, the S. aureus phage DZ25 was isolated from milk and the lysin LysDZ25 with excellent tolerance to serum and NaCl solution was identified. Subsequently, to improve the lytic activity and thermal stability of LysDZ25, RoseTTAFold was used to construct threedimensional (3D) structures, molecular dynamics (MD) simulation was used for conformational acquisition, and the MDL strategy previously developed in our lab was used to rationally design variants. After two rounds of rational design, the optimal variant with improved thermal stability, S333V/N245R/D299L, was obtained, and its half-life time was 4.0-fold that of wild-type LysDZ25. At 37, 40, 45, and 50 degrees C, the lytic activity of the optimal triple-point variant S333V/N245R/ D299L was increased by 17.3-, 26.7-, 20.2-, and 50.1-fold compared with that of the wild-type LysDZ25, respectively. Finally, cell count was used to evaluate the lytic activity, and the results showed that the optimal variant S333V/N245R/D299L could drop about 3.5 log 10 values compared with the control and about 2.6 log 10 values compared with the wild-type LysDZ25.
- 相关文献
作者其他论文 更多>>
-
Recombinase polymerase amplification combined with lateral flow biosensor for rapid visual detection of Clostridium perfringens in chicken meat and milk
作者：Tian, Rui;Xie, Feng;Liu, Guangjin;Zhang, Wei;Tian, Rui;Xie, Feng;Liu, Guangjin;Zhang, Wei;Liu, Yuqing;Li, Qingxia;Wang, Jinxiu;Dai, Lei;Zhang, Hongjian
关键词：foodborne pathogen; Clostridium perfringens; on-site detection method; recombinase polymerase amplification; lateral flow biosensor
-
Salmonella enteritidis acquires phage resistance through a point mutation in rfbD but loses some of its environmental adaptability
作者：Zeng, Yukun;Li, Ping;Liu, Shenglong;Shen, Mangmang;Zhou, Xin;Zeng, Yukun;Zhou, Xin;Zeng, Yukun;Zhou, Xin;Liu, Yuqing
关键词：Salmonella enteritidis; rfbD gene; point mutation; phage resistance; environmental adaptability
-
Antimicrobial Resistance and Molecular Characterization of Escherichia coli from Healthy Chickens in Shandong, China from 2009 to 2014
作者：Li, Lulu;Zhao, Xiaonan;Zhang, Qing;Luo, Yanbo;Hu, Ming;Chen, Yibao;Liu, Yuqing;Li, Lulu;Zhao, Xiaonan;Zhang, Qing;Luo, Yanbo;Hu, Ming;Chen, Yibao;Liu, Yuqing;Guo, Genglin
关键词：Escherichia coli; antimicrobial resistance; ESBL; PMQR; PMCR
-
Toxin gene detection and antibiotic resistance of Clostridium perfringens from aquatic sources
作者：Li, Mengxuan;Wang, Ying;Hou, Bingyu;Cai, Yumei;Chen, Yibao;Hu, Ming;Zhao, Xiaonan;Zhang, Qing;Li, Lulu;Luo, Yanbo;Liu, Yuqing
关键词：Aquatic animals; Clostridium perfringens; Toxin gene; Antibiotic resistance phenotype; Clam
-
Efficacy of a new K3-specific bacteriophage for controlling Klebsiella pneumoniae in milk and its potential to disrupt biofilm formation
作者：Li, Pei;Zhou, Yu;Qin, Xiayan;Hu, Zimeng;Ma, Jiale;Zhang, Wei;Li, Pei;Zhou, Yu;Hu, Zimeng;Zhang, Wei;Guo, Genglin;Li, Min;Tan, Zhongming;Liu, Yuqing;Han, Xiangan;Du, Hong;Zhang, Wei
关键词：Klebsiella pneumoniae; Milk; Bacteriophage; K3; Biofilm; Depolymerase
-
Genomic analysis and experimental pathogenic characterization of Riemerella anatipestifer isolates from chickens in China
作者：Chen, Yibao;Liu, Zhengjie;Hu, Ming;Luo, Yanbo;Zhang, Qing;Li, Lulu;Zhao, Xiaonan;Zhao, Min;Liu, Yuqing;Li, Xiaojing;Luo, Yanbo;Liu, Wenhua;Ma, Jinxiang
关键词：R. anatipestifer; genome analysis; infection chicken; jelly-like; vertically
-
Development of the CRISPR-Cas12a system for editing of Pseudomonas aeruginosa phages
作者：Chen, Yibao;Yan, Bingjie;Zhang, Xue;Liu, Zhengjie;Zhang, Qing;Li, Lulu;Hu, Ming;Zhao, Xiaonan;Xu, Xiaohui;Lv, Qianghua;Luo, Yanbo;Liu, Yuqing;Chen, Yibao;Yan, Bingjie;Zhang, Xue;Liu, Zhengjie;Zhang, Qing;Li, Lulu;Hu, Ming;Zhao, Xiaonan;Xu, Xiaohui;Lv, Qianghua;Luo, Yanbo;Liu, Yuqing;Chen, Weizhong;Cai, Yumei;Chen, Yibao;Hu, Ming;Liu, Yuqing
关键词：
