Quantification of Free Short-Chain Fatty Acids in Raw Cow Milk by Gas Chromatography-Mass Spectrometry
文献类型： 外文期刊
作者： Wu, Xufang 1 ; Wang, Fengen 4 ; Chen, Meiqing 1 ; Wang, Jiaqi 1 ; Zhang, Yangdong 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Lab Qual & Safety Risk Assessment Dairy Prod, Minist Agr & Rural Affairs, Beijing 100193, Peoples R China
2.Chinese Acad Agr Sci, Inst Anim Sci, Milk & Dairy Prod Inspect Ctr, Minist Agr & Rural Affairs, Beijing 100193, Peoples R China
3.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
4.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Peoples R China
关键词： milk; free short-chain fatty acids; gas chromatography-mass spectrometry
期刊名称：FOODS （ 影响因子：5.2； 五年影响因子：5.5 ）
ISSN：
年卷期： 2023 年 12 卷 7 期
页码：
收录情况： SCI
摘要： Free short-chain fatty acids (FSCFAs) are a momentous contributor to the flavor of the raw cow milk. Hence, the purpose of this research was to build an approach for the quantification of 10 FSCFAs in raw cow milk. Raw cow milk samples are acidified by hydrochloric acid ethanol (0.5%) solution pretreatment and then processed on the gas chromatography-mass spectrometry. With the exception of iso C5:0 and anteiso C5:0 co-flux, the remaining eight FSCFAs were effectively separated by chromatography. The methodological validation data revealed that the linear relationship satisfied the assay requirements (coefficient of determination >0.999), the limits of quantification were 0.167 to 1.250 mu g mL(-1), the recoveries ranged from 85.62% to 126.42%, the coefficients of variation were 1.40 similar to 12.15%, and no SCFAs in the triglyceride form were potential degradation, and the precision ranging from 0.56% to 9.09%. Our easy, fast, and robust method successfully determined three FSCFAs in raw cow milk without derivatization. Some characteristic features of FSCFAs have been discovered in raw cow milk such as its higher percentages of C4:0 and C6:0. Our research has provided a very valuable method for the future quality and safety control of raw milk and nutritional studies.
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