Rapid determination of furosine in milk using microwave-assisted HCl hydrolysis and ultra-performance liquid chromatography
文献类型： 外文期刊
作者： Wang, Fengen 1 ; Zhang, Yangdong 1 ; Wen, Fang 1 ; Zheng, Nan 1 ; Wang, Jiaqi 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Key Lab Qual & Safety Control Milk & Dairy Prod, Beijing 100193, Peoples R China
2.Xinjiang Agr Univ, Coll Anim Sci, Urumqi 830091, Peoples R China
3.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Peoples R China
4.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Lab Qual & Safety Risk Assessment Dairy Prod, Beijing 100193, Peoples R China
关键词： furosine; milk; microwave-assisted HCl hydrolysis; ultra-performance liquid chromatography
期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）
ISSN： 0022-0302
年卷期： 2020 年 103 卷 12 期
页码：
收录情况： SCI
摘要： Because the conventional methods for furosine analy-sis are time-consuming, a modified method is presented to improve analysis efficiency. Microwave-assisted HCl hydrolysis was performed at 140, 150, and 160 degrees C for 10 to 200 min, with 6, 8, or 9 M HCl. The hydrolysate purification process was carried out using only paper and membrane filtration. An ultra-performance liquid chromatography (UPLC) system was used to achieve rapid analysis of furosine. The results showed that microwave-assisted HCl hydrolysis at 8 M and 160 degrees C led to a stable furosine yield and took only 40 min. The UPLC analysis was completed in 8 min. The modified method was validated and obtained limit of detection at 3 mu g/L, limit of quantitation of 10 mu g/L, linearity range of 0.2 to 5.0 mg/L, 80.5 to 94.2% recoveries from spiked samples, and coefficients of variation of 2.2 to 6.8%. The modified method is rapid and reliable for the determination of furosine in milk.
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