A new isopropyl esterification method for quantitative profiling of short-chain fatty acids in human and cow milk by gas chromatograph-mass spectrometer
文献类型： 外文期刊
作者： Wu, Xufang 1 ; Chen, Meiqing 1 ; Wang, Fengen 4 ; Si, Boxue 1 ; Pan, Junyu 5 ; Yang, Jiyong 1 ; Wang, Jiaqi 1 ; Zhang, Yangdong 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Key Lab Qual & Safety Control Milk & Dairy Prod, Beijing 100193, Peoples R China
2.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Lab Qual & Safety Risk Assessment Dairy Prod, Beijing 100193, Peoples R China
3.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
4.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Peoples R China
5.Qingdao Agr Univ, Coll Food Sci & Engn, Qingdao 266109, Peoples R China
关键词： short-chain fatty acids; isopropyl ester; gas chromatography-mass spectrometry; milk
期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：3.7； 五年影响因子：4.1 ）
ISSN： 0022-0302
年卷期： 2024 年 107 卷 8 期
页码：
收录情况： SCI
摘要： Short-chain fatty acids (SCFA) content in milk may have been underestimated due to the neglect of the esterified SCFA content and the lack of an accurate detection method, especially for C1:0, C2:0, and C3:0 SCFA. In this study, an accurate GC-MS profiling method was established for 10 SCFA. A 2-step esterification, including alkaline saponification (60 degrees C for 30 min) and acid- catalyzed esterification (80 degrees C for 150 min) in water/ isopropyl/hexane (1:2:1, volume ratio), was found to be the most suitable for the quantification of esterified and nonesterified SCFA analysis. The validation results demonstrate satisfactory linearity, sensitivity, matrix effects, precision, and accuracy. The recoveries of nonesterified and esterified SCFA ranged from 82.78% to 112.49%, respectively. Human milk is distinguished from cow milk by its higher C1:0 and C2:0 content and lower C4:0 and C6:0 content. This method successfully accomplished qualitative and quantitative estimation of all 10 SCFA in milk, including both nonesterified and esterified SCFA. Furthermore, whether our method is applicable for the determination of SCFA in serum, rumen fluid, and feces remains to be explored.
- 相关文献
作者其他论文 更多>>
-
Label-Free quantitation of milk oligosaccharides from different mammal species and heat treatment influence
作者：Yao, Qianqian;Gao, Yanan;Wang, Jiaqi;Zheng, Nan;Yao, Qianqian;Delcenserie, Veronique;Wang, Fengen
关键词：Milk oligosaccharides (MOs); LC-ESI-MS/MS; Human milk; Domestic animal milk; Heat treatment
-
Quantification of Free Short-Chain Fatty Acids in Raw Cow Milk by Gas Chromatography-Mass Spectrometry
作者：Wu, Xufang;Chen, Meiqing;Wang, Jiaqi;Zhang, Yangdong;Wu, Xufang;Chen, Meiqing;Wang, Jiaqi;Zhang, Yangdong;Wu, Xufang;Chen, Meiqing;Wang, Jiaqi;Zhang, Yangdong;Wang, Fengen
关键词：milk; free short-chain fatty acids; gas chromatography-mass spectrometry
-
Updating the fatty acid profiles of retail bovine milk in China based on an improved GC-MS method: implications for nutrition
作者：Chen, Meiqing;Wu, Xufang;Si, Boxue;Pan, Junyu;Zheng, Nan;Zhang, Yangdong;Wang, Jiaqi;Chen, Meiqing;Wu, Xufang;Si, Boxue;Pan, Junyu;Zheng, Nan;Zhang, Yangdong;Wang, Jiaqi;Wang, Fengen
关键词：gas chromatography-mass spectrometry; fatty acid profile; retail milk survey; dietary intakes; human health
-
Fatty acid profiles of milk from Holstein cows, Jersey cows, buffalos, yaks, humans, goats, camels, and donkeys based on gas chromatography-mass spectrometry
作者：Wang, Fengen;Wang, Jiaqi;Wang, Fengen;Chen, Meiqing;Luo, Runbo;Huang, Guoxin;Wu, Xufang;Zheng, Nan;Zhang, Yangdong;Wang, Jiaqi;Wang, Fengen;Wang, Fengen;Chen, Meiqing;Luo, Runbo;Huang, Guoxin;Wu, Xufang;Zheng, Nan;Zhang, Yangdong;Wang, Jiaqi
关键词：milk fatty acid; nonruminant; ruminant; Key fatty acid; gas chromatography-mass spectrometry
-
Simultaneous Determination of C18 Fatty Acids in Milk by GC-MS
作者：Chen, Meiqing;Zhang, Yangdong;Wang, Fengen;Zheng, Nan;Wang, Jiaqi;Chen, Meiqing;Zhang, Yangdong;Wang, Fengen;Zheng, Nan;Wang, Jiaqi;Chen, Meiqing;Zhang, Yangdong;Wang, Fengen;Zheng, Nan;Wang, Jiaqi;Chen, Meiqing;Zhang, Yangdong;Zheng, Nan;Wang, Jiaqi;Wang, Fengen
关键词：determination; fatty acids; milk; GC-MS
-
Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging Mice
作者：Wang, Fengen;Deng, Ligang;Wang, Lei;Zhou, Hongbin;Chen, Jingqing;Zhou, Xihong
关键词：
-
Rapid determination of furosine in milk using microwave-assisted HCl hydrolysis and ultra-performance liquid chromatography
作者：Wang, Fengen;Zhang, Yangdong;Wen, Fang;Zheng, Nan;Wang, Jiaqi;Wang, Fengen;Wang, Jiaqi;Wang, Fengen;Wang, Fengen;Zhang, Yangdong;Wen, Fang;Zheng, Nan;Wang, Jiaqi
关键词：furosine; milk; microwave-assisted HCl hydrolysis; ultra-performance liquid chromatography
