您好，欢迎访问山东省农业科学院文献资源数据库平台

Simultaneous Determination of C18 Fatty Acids in Milk by GC-MS

文献类型： 外文期刊

作者： Chen, Meiqing 1 ; Zhang, Yangdong 1 ; Wang, Fengen 1 ; Zheng, Nan 1 ; Wang, Jiaqi 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Key Lab Qual & Safety Control Milk & Dairy Prod, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Lab Qual & Safety Risk Assessment Dairy Prod, Beijing 100193, Peoples R China

3.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Milk & Milk Prod Inspect Ctr, Beijing 100193, Peoples R China

4.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

5.Shandong Acad Agr Sci, Inst Qual Standard & Testing Technol Agroprod, Jinan 250100, Peoples R China

关键词： determination; fatty acids; milk; GC-MS

期刊名称：SEPARATIONS （ 影响因子：2.777； ）

ISSN：

年卷期： 2021 年 8 卷 8 期

页码：

收录情况： SCI

摘要： The determination of C18 fatty acids (FAs) is a key and difficult aspect in FA profiling, and a qualified method with good chromatographic separation and high sensitivity, as well as easy methylation, is required. A GC-MS method was established to simultaneously determine C18 FAs in milk. To simplify the methylation protocol for milk samples, besides a base-catalyzation methylation (50 degrees C for 20 min), the necessity of an additional acid-catalyzation was also studied using different temperatures (60 degrees C, 70 degrees C, 80 degrees C, and 90 degrees C) and durations (90 min and 150 min). The results showed that the chromatographic resolution was improved, although three co-eluted peaks existed. The base-catalyzation was sufficient, and an additional acid-catalyzation was not necessary. The proposed method was validated with good sensitivity, linearity, accuracy, and precision, and then applied in determining C18 FAs in 20 raw milk and 30 commercial milk samples. UHT milk presented a different profile of C18 FAs from raw milk and PAS milk samples, which indicated that excessive heating could change the profile. Overall, the proposed method is a high-throughput and competent approach for the determination of C18 FAs in milk, and which presents an improvement in chromatographic resolution and sensitivity, as well as a simplification of methylation.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台