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Increased expression of a novel splice variant of the complement component 4 (C4A) gene in mastitis-infected dairy cattle

文献类型： 外文期刊

作者： Yang, Y. 1 ; Huang, J. M. 1 ; Ju, Z. H. 1 ; Li, Q. L. 1 ; Zhou, L. 1 ; Li, R. L. 1 ; Li, J. B. 1 ; Shi, F. X. 2 ; Zhong, J. 1 ;

作者机构： 1.Shandong Acad Agr Sci, Dairy Cattle Sci Res Ctr, Jinan, Peoples R China

2.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing, Jiangsu, Peoples R China

3.State Intellectual Property Off, Patent Examinat & Cooperat Ctr Jiangsu, Suzhou, Peoples R China

关键词： C4A gene;Alternative splicing;Transcription pattern;Dairy cattle;Mastitis

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2012 年 11 卷 3 期

页码：

收录情况： SCI

摘要： The complement system helps in the direct lysis of invading pathogens and modulates phagocytic, humoral and cellular immune responses. Complement 4 is a critical component in complement activity and protection against many bacterial pathogens because it is essential to classical and lectin activation pathways. We used reverse transcription and PCR to investigate alternative splicing and expression of the complement component 4 (C4A) gene in Chinese Holstein cattle. The PCR products were cloned and sequenced. A novel splice variant involving intron 10 was identified, which we named C4A-AS. To examine how C4A gene activity is affected by bovine mastitis, six Chinese Holstein cattle were divided into healthy (non-mastitic) and Staphylococcus aureus-induced mastitic groups. Real-time quantitative PCR (qRT-PCR) revealed that the C4A-complete and C4A-AS transcripts are expressed at significantly different levels in healthy cows, while there were no significant differences in the mastitic group (P = 0.257). Expression of C4A-AS increased significantly when mastitis developed. We also examined the expression of C4A-complete and C4A-AS in several tissues (liver, heart, spleen, lung, kidney, tongue, and muscle). The two transcripts were expressed in all of these tissues but there were no significant differences in expression between healthy and mastitic cows. We therefore conclude that the C4A-complete transcript is the main transcript under normal physiological conditions, while C4A-AS is augmented when mastitis develops.

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