A SNP in the 3 '-UTR of HSF1 in dairy cattle affects binding of target bta-miR-484
文献类型： 外文期刊
作者： Li, Q. L. 1 ; Zhang, Z. F. 2 ; Xia, P. 1 ; Wang, Y. J. 1 ; Wu, Z. Y. 1 ; Jia, Y. H. 1 ; Chang, S. M. 1 ; Chu, M. X. 4 ;
作者机构： 1.Langfang Teachers Univ, Edible & Med Fungi Res & Dev Ctr, Coll Life Sci, Langfang, Peoples R China
2.Langfang Agr Bur, Vet Adm Off, Langfang, Peoples R China
3.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan, Peoples R China
4.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr, Key Lab Farm Anim Genet Resources & Germplasm Inn, Beijing 100193, Peoples R China
关键词： Dairy cattle; HSF1; bta-miR-484; Thermo tolerance; Target; Single nucleotide polymorphism
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2015 年 14 卷 4 期
页码：
收录情况： SCI
摘要： The heat shock transcription factor 1 gene (HSF1) plays a key role in the heat stress response. We previously found a single nucleotide polymorphism (SNP) in the 3'-untranslated region (g.4693G>T) of HSF1 that was related to thermo tolerance in Chinese Holstein cattle through association analysis. However, it is not known whether other SNPs also affect thermo tolerance. In this study a novel SNP, g.1451G>T, was identified by DNA sequencing and genotyped using creating restriction site-polymerase chain reaction methodology. The g.1451G>T polymorphic site met Hardy-Weinberg equilibrium (P > 0.05). Association analysis demonstrated that this SNP had no effect on thermo tolerance traits in Holstein cattle. Findings of the study compared to the analysis of g.4693G>T further indicated that g.4693 G>T may play an important role in thermo tolerance, although the mechanism is not clear. RNA hybrid and Targetscan prediction showed that the minimum free energy hybridization of bta-miR-484 with HSF1 3'-UTR was -31.9 kcal/mol and g.4693 G>T was in the seed sequence of bovine HSF1 that binds to bta-miR-484. Analysis by Luciferase assay indicated that HSF1 expression was directly targeted by bta-miR-484 in HEK 293T cells, and the Rluc/luc ratio of wildtype (GG) was lower than that of the mutant (TT) (P < 0.05). These results suggest that g.4693 G>T affects binding of HSF1 to bta-miR-484.
- 相关文献
作者其他论文 更多>>
-
Genome-wide association study of body weight and conformation traits in neonatal sheep
作者：Tao, L.;He, X. Y.;Chu, M. X.;Pan, L. X.;Wang, J. W.;Gan, S. Q.
关键词：birth weight; body conformation; candidate gene; GWAS; sheep; SNPs
-
A SNP in the 3 '-UTR of HSF1 in dairy cattle affects binding of target bta-miR-484
作者：Li, Q. L.;Xia, P.;Wang, Y. J.;Wu, Z. Y.;Jia, Y. H.;Chang, S. M.;Zhang, Z. F.;Li, Q. L.;Chu, M. X.
关键词：Dairy cattle;HSF1;bta-miR-484;Thermo tolerance;Target;Single nucleotide polymorphism
-
Identification and characterization of a novel splice variant of the PLC zeta 1 gene in bull testis tissues
作者：Ju, Z. H.;Pan, Q.;Zhang, Y.;Huang, J. M.;Qi, C.;Wang, X. G.;Li, Q. L.;Zhong, J. F.;Wang, C. F.;Pan, Q.;Liu, M.
关键词：PLC zeta 1 gene;Chinese Holstein bull;Splice variant;Quantitative real-time polymerase chain reaction;Protein sequencing analysis
-
Identification and characterization of a novel splice variant of the PLC zeta 1 gene in bull testis tissues
作者：Ju, Z. H.;Pan, Q.;Zhang, Y.;Huang, J. M.;Qi, C.;Wang, X. G.;Li, Q. L.;Zhong, J. F.;Wang, C. F.;Pan, Q.;Liu, M.
关键词：PLC zeta 1 gene; Chinese Holstein bull; Splice variant; Quantitative real-time polymerase chain reaction; Protein sequencing analysis
-
Increased expression of a novel splice variant of the complement component 4 (C4A) gene in mastitis-infected dairy cattle
作者：Yang, Y.;Huang, J. M.;Ju, Z. H.;Li, Q. L.;Zhou, L.;Li, R. L.;Li, J. B.;Zhong, J. F.;Wang, C. F.;Yang, Y.;Shi, F. X.;Yang, Y.
关键词：C4A gene;Alternative splicing;Transcription pattern;Dairy cattle;Mastitis
-
Novel SNPs of the mannan-binding lectin 2 gene and their association with production traits in Chinese Holsteins
作者：Zhao, Z. L.;Zhang, J. B.;Zhao, Z. L.;Wang, C. F.;Li, Q. L.;Ju, Z. H.;Huang, J. M.;Li, J. B.;Zhong, J. F.
关键词：Bovine;MBL2;SNPs;Somatic cell score
-
Increased expression of a novel splice variant of the complement component 4 (C4A) gene in mastitis-infected dairy cattle
作者：Yang, Y.;Huang, J. M.;Ju, Z. H.;Li, Q. L.;Zhou, L.;Li, R. L.;Li, J. B.;Zhong, J. F.;Wang, C. F.;Yang, Y.;Shi, F. X.;Yang, Y.
关键词：C4A gene; Alternative splicing; Transcription pattern; Dairy cattle; Mastitis
