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Identification and characterization of a novel splice variant of the PLC zeta 1 gene in bull testis tissues

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文献类型：外文期刊

作者： Ju, Z. H. ¹ ; Pan, Q. ¹ ; Zhang, Y. ¹ ; Huang, J. M. ¹ ; Qi, C. ¹ ; Wang, X. G. ¹ ; Li, Q. L. ¹ ; Zhong, J. F. ¹ ; Liu, M. ² ;

作者机构： 1.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan, Peoples R China

2.Nanjing Normal Univ, Coll Life Sci, Nanjing, Jiangsu, Peoples R China

关键词： PLC zeta 1 gene;Chinese Holstein bull;Splice variant;Quantitative real-time polymerase chain reaction;Protein sequencing analysis

期刊名称：GENETICS AND MOLECULAR RESEARCH （影响因子：0.764；五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2014 年 13 卷 4 期

页码：

收录情况： SCI

摘要： Phospholipase C zeta 1 (PLC zeta 1), which transcribes a key protein, has an important function in oocyte activation and embryo development because PLC zeta 1 can trigger a series of intracellular Ca2+ oscillations in mammals. In this study, a novel splice variant in the testis tissues of adult and fetal Chinese Holstein bulls was characterized by reverse transcription-polymerase chain reaction (RT-PCR) and sequencing analysis. The novel splice variant PLC zeta 1-sv1 was derived from the PLC zeta 1 complete transcript (PLC zeta 1-complete) by alternative splicing; the alternative splicing pattern exhibited alternative 5'-splice sites. The full-length transcript, PLC zeta 1-complete, is the main transcript found in fetal and adult cow testis tissue. Quantitative real-time PCR (qPCR) analysis demonstrated that the expression levels of the PLC zeta 1-complete transcript were significantly higher than those of the PLC zeta 1-sv1 splice variant in bovine testis tissues. PLC zeta 1 protein sequencing analysis showed that the amino acids at positions 453 to 457 were deleted in PLC zeta 1-sv1, thereby terminating transcription prematurely. In summary, this study provided information to elucidate the structure and function of the bovine PLC zeta 1 gene.

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