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A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression

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文献类型：外文期刊

作者： Li, H. ¹ ; Jiang, Q. ¹ ; Ju, Z. H. ¹ ; Huang, J. M. ¹ ; Wang, X. G. ¹ ; Yang, C. H. ¹ ; Sun, Y. ¹ ; Yang, G. W. ² ; Zhong, J ¹ ;

作者机构： 1.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan, Shandong, Peoples R China

2.Shandong Normal Univ, Coll Life Sci, Jinan, Peoples R China

关键词： Alternative splicing;Chinese Holstein bull;Testis;GALNTL5 gene;Quantitative real-time polymerase chain reaction

期刊名称：GENETICS AND MOLECULAR RESEARCH （影响因子：0.764；五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 2 期

页码：

收录情况： SCI

摘要： The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is a newly identified protein that is specifically expressed in testis tissue and participates in spermatogenesis. In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain reaction (RT-PCR) and clone sequencing methods. The novel GALNTL5 isoform was derived from the complete transcript, GALNTL5-complete, via alternative splicing (AS). The pattern of the splice variant was exon skipping. Bovine GALNTL5 transcripts were expressed in the testis, as demonstrated by RT-PCR. The expression levels of both transcripts were higher in adult testes than in calf testes (P < 0.05). In addition, prediction analysis showed that the GALNTL5-AS transcript only encoded 122 amino acids and lost its glycosyltransferase 1 and Gal/GalNAc-T motifs, which may result in a dysfunctional protein compared with the predominant transcript GALNTL5-complete. This study improves our understanding of the bovine GALNTL5 gene function during bull sperm formation.

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