A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression
文献类型： 外文期刊
作者： Li, H. 1 ; Jiang, Q. 1 ; Ju, Z. H. 1 ; Huang, J. M. 1 ; Wang, X. G. 1 ; Yang, C. H. 1 ; Sun, Y. 1 ; Yang, G. W. 2 ; Zhong, J. F. 1 ; Wang, C. F. 1 ;
作者机构： 1.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan, Shandong, Peoples R China
2.Shandong Normal Univ, Coll Life Sci, Jinan, Peoples R China
关键词： Alternative splicing; Chinese Holstein bull; Testis; GALNTL5 gene; Quantitative real-time polymerase chain reaction
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2016 年 15 卷 2 期
页码：
收录情况： SCI
摘要： The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is a newly identified protein that is specifically expressed in testis tissue and participates in spermatogenesis. In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain reaction (RT-PCR) and clone sequencing methods. The novel GALNTL5 isoform was derived from the complete transcript, GALNTL5-complete, via alternative splicing (AS). The pattern of the splice variant was exon skipping. Bovine GALNTL5 transcripts were expressed in the testis, as demonstrated by RT-PCR. The expression levels of both transcripts were higher in adult testes than in calf testes (P < 0.05). In addition, prediction analysis showed that the GALNTL5-AS transcript only encoded 122 amino acids and lost its glycosyltransferase 1 and Gal/GalNAc-T motifs, which may result in a dysfunctional protein compared with the predominant transcript GALNTL5-complete. This study improves our understanding of the bovine GALNTL5 gene function during bull sperm formation.
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A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression
作者：Li, H.;Jiang, Q.;Ju, Z. H.;Huang, J. M.;Wang, X. G.;Yang, C. H.;Sun, Y.;Zhong, J. F.;Wang, C. F.;Li, H.;Yang, G. W.
关键词：Alternative splicing;Chinese Holstein bull;Testis;GALNTL5 gene;Quantitative real-time polymerase chain reaction
