您好，欢迎访问山东省农业科学院文献资源数据库平台

Performance of Indirect ELISA for Maize Hybrid Purity Test

文献类型： 外文期刊

作者： Liu, Yuehui 1 ; Wang, Jun 1 ; Yang, XinChao 1 ; Sun, Naxin 1 ; Xu, Lihua 2 ;

作者机构： 1.Univ Jinan, Med & Life Sci Coll, Jinan, Peoples R China

2.Shandong Acad Agr Sci, Maize Res Inst, Jinan, Peoples R China

关键词： maize hybrid;indirect ELISA;purity test

期刊名称：ADVANCES IN CHEMICAL ENGINEERING III, PTS 1-4

ISSN： 1022-6680

年卷期： 2013 年 781-784 卷

页码：

收录情况： SCI

摘要： Maize is the second large crop in China that needed 100-120 million tons seeds for an average year and the production loss caused by inferior seeds was amazing. Bing an allogamous wind-pollinated plant, maize hybrid purity problem is mainly reflected the inbred lines seed mixed in hybrid. Therefore it is important to have specific and sensitive purity test methods to prevent the poor seeds into market. In this paper, we developed an inderect ELISA using polyconal antibody against maize inbred lines to bind extracts from whole grain with pure water. The single blind trial demonstrated that the average detectable rate of indrect ELISA was 95.2%. We also extended this method to other maize hybrid varieties, found it has no ability to descriminate the maize hybrid facticity.

  • 相关文献

[1]Development and Preliminary Application of an Indirect ELISA to Detect Infectious Bovine Rhinotracheitis Virus Using Recombinant Glycoprotein D of IBRV Strain SD. Song, Lingling,Zhang, Hui,Hou, Peili,Wang, Hongmei,Zhao, Guimin,He, Hongbin,Xia, Xianzhu. 2016

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台