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Delayed delivery of chromogenic substrate to nanozyme amplified aptamer lateral flow assay for acetamiprid

文献类型： 外文期刊

作者： Mao, Minxin 1 ; Sun, Fengxia 4 ; Wang, Jun 5 ; Li, Xiuping 1 ; Pan, Qiuli 5 ; Peng, Chifang 1 ; Wang, Zhouping 1 ;

作者机构： 1.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

3.Shandong Inst Pomol, Tai An 271018, Peoples R China

4.Shihezi Univ, Sch Food Sci & Technol, Shihezi 832000, Peoples R China

5.Shandong Inst Food & Drug Control, Jinan 250101, Peoples R China

关键词： Sugar barriers; Nanozyme; Substrate delayed delivery; Aptamer lateral flow assay; Acetamiprid

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）

ISSN：

年卷期： 2023 年 385 卷

页码：

收录情况： SCI

摘要： Nanozyme amplified lateral flow assay (LFA) demonstrated many attractive performances than the traditional gold nanoparticles (AuNPs) based LFA, however, they required several additional operations to implement the catalytic reaction of substrate. In this work, a chromogenic substrate integrated and nanozyme amplified aptamer lateral flow assay (CSNLFA) was constructed through automatic delivery of chromogenic substrate on strip. By introducing a sucrose-fixed chromogenic substrate loaded fiber (SCF) onto the sample pad of LFA strip, the CSNLFA platform achieved automated sequential catalytic reaction of substrate oxidation deposition after Au@Pt nanozyme was fully captured at T line of strip. This design was applied in an acetamiprid aptamer LFA and the developed aptamer CSNLFA (Apt-CSNLFA) achieved a linear range of 1-150 ng/mL for acetamiprid with the limit of detection (LOD) of 0.17 ng/mL. The Apt-CSNLFA demonstrated similar analytical performance to the conventional aptamer NLFAs but eliminated the additional operations for catalytic signal amplification. In addition, the new method was applied to the detection of tomato samples spiked with acetamiprid. The recovery ranged from 95% to 106.4% was obtained, which demonstrated the high application potential in rapid detection of acetamiprid.

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