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The host protein CALCOCO2 interacts with bovine viral diarrhoea virus Npro, inhibiting type I interferon production and thereby promoting viral replication

文献类型： 外文期刊

作者： Wang, Song 1 ; Wei, Ran 3 ; Ma, Xiaomei 2 ; Guo, Jin 2 ; Aizaz, Muhammad 2 ; Li, Fangxu 2 ; Wang, Jun 2 ; Wang, Hongmei 2 ; He, Hongbin 2 ;

作者机构： 1.Shandong Univ, Hosp 2, Dept Clin Lab, Jinan, Shandong, Peoples R China

2.Shandong Normal Univ, Coll Life Sci, Ruminant Dis Res Ctr, Jinan, Peoples R China

3.Shandong Acad Agr Sci, Poultry Inst, Jinan, Shandong, Peoples R China

关键词： BVDV; Npro; CALCOCO2; IRF3; IFN-I; innate immunity

期刊名称：VIRULENCE （ 影响因子：5.2； 五年影响因子：5.9 ）

ISSN： 2150-5594

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Bovine viral diarrhoea-mucosal disease caused by bovine viral diarrhoea virus (BVDV) is a major infectious disease that affects the cattle industry. The nonstructural protein Npro of BVDV antagonizes the type I interferon (IFN-I) pathway, thereby escaping the host immune response. The exact mechanism by which Npro uses host proteins to inhibit IFN-I production is unclear. The host protein CALCOCO2 was identified as a binding partner of Npro using a yeast two-hybrid screen. The interaction between Npro and CALCOCO2 was confirmed by yeast co-transformation, co-immunoprecipitation assays, and GST pull-down assays. The stable overexpression of CALCOCO2 markedly promoted BVDV propagation, while the knockdown of CALCOCO2 significantly inhibited BVDV replication in MDBK cells. Interestingly, CALCOCO2 inhibited IFN-I and IFN-stimulated gene production in BVDV-infected cells. Ectopic expression of CALCOCO2 effectively reduced IRF3 protein levels via the proteasome pathway. CALCOCO2 was found to promote Npro-mediated ubiquitination degradation of IRF3 by interacting with IRF3. Our results demonstrate the molecular mechanism by which Npro recruits the CALCOCO2 protein to regulate IRF3 degradation to inhibit IFN-I production.

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