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Preparation of Antibodies against Maize Inbred Lines Storage Proteins

文献类型： 外文期刊

作者： Liu, Yuehui 1 ; Wang, Jun 1 ; Yang, XinChao 1 ; Sun, Naxin 1 ; Xu, Lihua 2 ;

作者机构： 1.Univ Jinan, Medcine & Life Sci Coll, Jinan, Peoples R China

2.Shandong Acad Agr Sci, Maize Res Inst, Jinan, Peoples R China

关键词： maize inbred lines;storage proteins;anitody preparation

期刊名称：ADVANCES IN CHEMICAL ENGINEERING III, PTS 1-4

ISSN： 1022-6680

年卷期： 2013 年 781-784 卷

页码：

收录情况： SCI

摘要： A convenient way of producing effective antibody against maize seed storage proteins is present.The protein patterns of maize hybrid seed Ludan50 have two special protein bands with Polyacrylamide gel electrophoresis(PAGE) which was corresponding to its parent inbred lines Luyuan92 and Qi319. We purified the two proteins respectively by acid-PAGE and then immunized rabbit and laying hens, obtained the antiserums and yolk antibodies. Both antiserums and yolk antibodies have good specificity against the antigens, except that the yolk antibody has a higher titer. We also measured its specificity against different protein components extracted with pure water, 2.5% NaCl (w/v), 70%(v/v) alcohol, 10mM HCl and 50mM KOH. The results appears that the water-soluble proteins tended to be more specific than othors to the antibodies, so the two inbred lines can be identified from each other by ELISA with the antibody we have been produced.

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