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Alternative Soaking Media for the FDA Procedure in the Detection of Salmonella from Tomatoes and Spinach Leaf using Phage Magnetoelastic Biosensors

文献类型： 外文期刊

作者： Chen, I-Hsuan 1 ; Hu, Jiajia 2 ; Wang, Fengen 3 ; Horikawa, Shin 1 ; Barbaree, James M. 1 ; Chin, Bryan A. 1 ;

作者机构： 1.Auburn Univ, Auburn, AL 36849 USA

2.Changzhou Univ, Changzhou 213164, Jiangsu, Peoples R China

3.Shandong Acad Agr Sci, Jinan, Shandong, Peoples R China

关键词： Biosensors;wireless;Salmonella;fresh produce;soaking media;FDA procedure;Salmonella detection.Send correspondence to chenih1@auburn.edu

期刊名称：SENSING FOR AGRICULTURE AND FOOD QUALITY AND SAFETY VIII

ISSN： 0277-786X

年卷期： 2016 年 9864 卷

页码：

收录情况： SCI

摘要： Efforts were made to incorporate the phage Magnetoelastic (ME) biosensor in FDA's Spinach Soaking procedures according to FDA 2015 BAM method. Three soaking materials (Lactose broth, LB broth, and Peptone water) and various soaking times were investigated. Using merely 100 Salmonella cells spiked on the produce surfaces, the phage biosensors detected Salmonella within 5 hours when soaking tomatoes in LB broth as opposed to taking up to 24 hours. Salmonella was detected on spinach leaves within 7 hours. These phage ME biosensors provide a promising rapid detection platform using LB broth in FDA's soaking procedures while shortening the incubation period.

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[1]Detection of Salmonella Typhimurium on Spinach Using Phage-Based Magnetoelastic Biosensors. Wang, Fengen,Horikawa, Shin,Wikle, Howard C., III,Du, Songtao,Liu, Yuzhe,Chin, Bryan A.,Hu, Jiajia,Chen, I-Hsuan. 2017

[2]Serotype distribution and antibiotic resistance of Salmonella in food-producing animals in Shandong province of China, 2009 and 2012. Lai, Jing,Wu, Congming,Wu, Chenbin,Wang, Yang,Wang, Hongyu,Shen, Jianzhong,Qi, Jing,Liu, Yuqing. 2014

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