您好，欢迎访问山东省农业科学院文献资源数据库平台

Overcoming obstacles to interspecific hybridization between Gossypium hirsutum and G. turneri

文献类型： 外文期刊

作者： Chen, Yu 1 ; Chen, Yu 1 ; Feng, Shouli 2 ; Zhao, Ting; Zhou, Baoliang;

作者机构： 1.Nanjing Agr Univ, MOE Hybrid Cotton R&D Engn Res Ctr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China; Inst Bot, Nanjing 210014, Jiangsu, Peoples R China; Chinese Acad Sci, Nanjing 210014, Jiangsu, Peoples R China; Shandong Acad Agr Sci, Minist Agr, Cotton Res Ctr, Key Lab Cotton Breeding & Cultivat Huang Huai Hai, Jinan 250100, Shandong, Peoples R China

2.Nanjing Agr Univ, MOE Hybrid Cotton R&D Engn Res Ctr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China; Inst Bot, Nanjing 210014, Jiangsu, Peoples R China; Chinese Acad Sci, Nanjin

关键词： Chromosome doubling;Embryo rescue;Gossypium hirsutum;Interspecific hybridization;Molecular marker;Wild cotton

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2018 年 214 卷 2 期

页码：

收录情况： SCI

摘要： Gossypium turneri, a wild cotton species (2n = 2X = 26, D10D10) originating from Mexico, possesses invaluable characteristics unavailable in the cultivated tetraploid cotton gene pool, such as caducous involucels at anthesis, resistance to insects and tolerance to abiotic stresses. However, transferring desired characteristics from wild species into cultivated cotton is often fraught with diverse obstacles. Here, Gossypium hirsutum (as the maternal parent) and G. turneri were crossed in the Hainan Province of China, and the obtained hybrid seeds (2n = 3X = 39, ADD(10)) were treated with 0.075% colchicine solution for 48 h to double the chromosome complement in order to overcome triploid F-1 sterility and to generate a fertile hexaploid. Chromosome doubling was successful in four individuals. However, the new synthetic hexaploids derived from these individuals were still highly sterile, and no seeds were generated by selfing or crossing. Therefore, an embryo rescue technique was employed in an attempt to produce progenies from the new synthetic hexaploids. Consequently, a total of six large embryos were obtained on MSB2K medium supplemented with 0.5 mg l(-1) KIN and 250 mg l(-1) CH using ovules from backcrossing that were 3 days post-anthesis. Four grafted surviving seedlings were confirmed to be the progenies (pentaploids) of the new synthetic hexaploids using cytological observations and molecular markers. Eight putative fertile individuals derived from backcrossing the above pentaploids were confirmed using SSR markers and generated an abundance of normal seeds. This research lays a foundation for transferring desirable characteristics from G. turneri into upland cotton.

  • 相关文献

[1]Creation of the technique of interspecific hybridization for breeding in cotton. Liang, ZL,Jiang, RQ,Zhong, WN,He, JX,Sun, CW,Qou, ZJ,Liu, DL,Zhang, XX,Zhao, GZ,Niu, YZ,Wang, JD,Wang, Y,Liang, LM,Wang, LM. 2002

[2]Review of Walnut Breeding Research at the Shandong Institute of Pomology. Zhang, M. Y.,Xu, Y.,Xiang, K.,Wang, X. L..

[3]Heterosis in yield, endotoxin expression and some physiological parameters in Bt transgenic cotton. Dong, H. Z.,Li, W. J.,Tang, W.,Li, Z. H.,Zhang, D. M.. 2007

[4]Characterization of eleven monosomic alien addition lines added from Gossypium anomalum to Gossypium hirsutum using improved GISH and SSR markers. Wang, Xiaoxiao,Wang, Yingying,Wang, Chen,Chen, Yu,Chen, Yu,Feng, Shouli,Zhao, Ting,Zhou, Baoliang,Chen, Yu. 2016

[5]Inducement and identification of chromosome introgression and translocation of Gossypium australe on Gossypium hirsutum. Wang, Yingying,Feng, Shouli,Li, Sai,Tang, Dong,Chen, Yu,Chen, Yu,Zhou, Baoliang,Chen, Yu,Chen, Yu. 2018

[6]Quantitative trait loci mapping for yield and its components by using two immortalized populations of a heterotic hybrid in Gossypium hirsutum L.. Liu, Renzhong,Wang, Baohua,Guo, Wangzhen,Qin, Yongsheng,Zhang, Yuanming,Zhang, Tianzhen,Liu, Renzhong,Wang, Liguo. 2012

[7]Feeding behavioral response of cotton aphid, Aphis gossypii, to elevated CO2: EPG test with leaf microstructure and leaf chemistry. Jiang, Shoulin,Li, Teng,Chen, Fajun,Liu, Tongjin,Yu, Fulan,Parajulee, Megha N.,Zhang, Limin.

[8]Peanut (Arachis hypogaea L.) Omics and Biotechnology in China. Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Liu, Shuan-Tao. 2011

[9]Agronomic Traits and Molecular Marker Identification of Wheat-Aegilops caudata Addition Lines. Gong, Wenping,Han, Ran,Li, Haosheng,Song, Jianmin,Li, Genying,Liu, Aifeng,Cao, Xinyou,Guo, Jun,Zhai, Shengnan,Cheng, Dungong,Zhao, Zhendong,Liu, Cheng,Liu, Jianjun,Yan, Hongfei,Li, Genying,Cao, Xinyou,Liu, Cheng. 2017

[10]Cytogenetic and molecular markers for detecting Aegilops uniaristata chromosomes in a wheat background. Gong, Wenping,Li, Guangrong,Zhou, Jianping,Liu, Cheng,Yang, Zujun,Li, Genying,Liu, Cheng,Huang, Chengyan,Zhao, Zhendong.

[11]Molecular and Cytogenetic Characterization of a Powdery Mildew-Resistant Wheat-Aegilops mutica Partial Amphiploid and Addition Line. Liu, Cheng,Gong, Wen-Ping,Li, Gen-Ying,Han, Ran,Li, Hao-Sheng,Song, Jian-Min,Liu, Ai-Feng,Cao, Xin-You,Chu, Xiu-Sheng,Huang, Cheng-Yan,Zhao, Zhen-Dong,Liu, Jian-Jun,Li, Guang-Rong,Yang, Zu-Jun.

[12]Analysis of the MdMYB1 gene sequence and development of new molecular markers related to apple skin color and fruit-bearing traits. Yuan, Kejun,Wang, Changjun,Xin, Li,Zhou, Guangfang,Li, Linguang,Shen, Guangning,Wang, Jianghui.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台