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Day length affects flowering time through regulating circadian oscillator and its downstream genes in photoperiod pathway in Gossypium hirsutum

文献类型： 外文期刊

作者： Pan, Ao 1 ; Yuan, Yang 4 ; Huo, Xuehan 2 ; Gao, Yang 2 ; Chen, Yu 2 ; Feng, Jiaojiao 3 ; Zhou, Zhonghua 1 ; Liu, Zhi 1 ; Wang, Furong 2 ; Zhang, Jun 2 ;

作者机构： 1.Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China

2.Shandong Acad Agr Sci, Inst Ind Crops, Key Lab Cotton Breeding & Cultivat Huang Huai Hai, Minist Agr & Rural Affairs, Jinan 250100, Peoples R China

3.Shandong Normal Univ, Life Sci Coll, Jinan 250358, Peoples R China

4.Heze Acad Agr Sci, Heze 274000, Peoples R China

关键词： Gossypium hirsutum L; Photoperiod; GhFKF1; Flowering; Virus induced gene silencing (VIGS)

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.6； 五年影响因子：5.7 ）

ISSN： 0926-6690

年卷期： 2024 年 220 卷

页码：

收录情况： SCI

摘要： Flowering time is an important improvement target in cotton breeding. The photoperiod is one of the significant factors affecting flowering in plant. Here, we conducted an exploration of how day length affects flowering and early maturating in upland cotton. After subjecting leaf samples of two cotton varieties, L19 (an early-maturity genotype) and L37 (a late-maturity genotype), to long-day (LD) and short-day (SD) conditions, we observed that the flowering time of both cotton varieties exhibited 7 - 11 days earlier under LD than SD. To investigate the underlying molecular mechanism, 8595 shared differentially expressed genes (DEGs) were identified in L19 and L37 based on transcriptome sequencing. Principal components analysis (PCA) showed the leaf stage and photoperiod treatment contributed the main differences between L19 and L37. KEGG analysis demonstrated that the DEGs were significantly enriched in circadian rhythms pathway and hormones signal transduction pathway, and the results of WGCNA further showed that green, darkgreen and lightgreen modules were significantly associated with day length. According to GO analysis, these DEGs in the three modules were involved in photoperiod related biological process such as rhythmic process, long-day photoperiodism and flowering process. A total of 75 hub genes were determined associated with each leaf developmental stage, and 8 genes were related to photoperiodic flowering pathway, including GhTOC1 , GhCCA1/LHY and GhFKF1 , etc. Finally, GhFKF1 , one of the hub genes, which highly expressed under long-day conditions, were selected for functional verification by virus induced gene silencing (VIGS). The results showed the flowering time of the silenced plants was delayed by 4.62 days. qPCR analysis indicated the expression of GhFKF1 in silenced plants was significantly reduced compared with the control and the expression levels of downstream genes GhFT and GhCO also decrease accordingly. Our research provides fresh insights into the molecular mechanisms of photoperiodic pathwaydepended flowering in cotton.

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