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An analysis of lncRNAs related to fiber quality and the discovery of their target genes in a Gossypium hirsutum line with Gossypium mustelinum introgression

文献类型： 外文期刊

作者： Wang, Baohua 1 ; Ji, Meijun 1 ; Fang, Hui 1 ; Gu, Haijing 1 ; Mehari, Teame Gereziher 1 ; Han, Jinlei 1 ; Feng, Wenxiang 1 ; Huo, Xuehan 2 ; Zhang, Jingxia 2 ; Chen, Yu 2 ; Zhang, Jun 2 ; Ditta, Allah 3 ; Khan, Muhammad K. R. 3 ; Paterson, Andrew H. 4 ; Chee, Peng W. 5 ; Wang, Kai 1 ;

作者机构： 1.Nantong Univ, Sch Life Sci, Nantong 226019, Jiangsu, Peoples R China

2.Shandong Acad Agr Sci, Inst Ind Crops, Key Lab Cotton Breeding & Cultivat Huang Huai Hai, Minist Agr & Rural Affairs China, Jinan 250100, Shandong, Peoples R China

3.Nucl Inst Agr & Biol, Plant Breeding & Genet Div, Faisalabad 38000, Pakistan

4.Univ Georgia, Plant Genome Mapping Lab, Athens, GA 30602 USA

5.Univ Georgia, Dept Crop & Soil Sci, Tifton, GA 31793 USA

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 2 期

页码：

收录情况： SCI

摘要： Key message Analysis of fiber quality lncRNAs and their target genes from a pair of Gossypium mustelinum near-isogenic lines provide new prospects for improving the fiber quality of Upland cotton. Long noncoding RNAs (lncRNAs) are an important part of genome transcription and play roles in a wide range of biological processes in plants. In this research, a pair of near-isogenic cotton lines, namely, a Gossypium mustelinum introgression line (IL9) with outstanding fiber quality and its recurrent Upland cotton parent (PD94042), were used as the experimental materials. Cotton fibers were selected for lncRNA sequencing at 17 and 21 days post-anthesis. A total of 2693 differentially expressed genes were identified. In total, 5841 lncRNAs were ultimately screened, from which 163 differentially expressed lncRNAs were identified. Target genes of the lncRNAs were predicted by two different methods: cis and trans. Some of the target genes were related to cell components, membrane components, plant hormone signal transduction and catalytic metabolism, and the results indicated that there might also be important effects on the development of fiber. Four differentially expressed target genes related to fiber quality (Gomus.D05G015100, Gomus.A05G281300, Gomus.A12G023400 and Gomus.A10G226800) were screened through gene function annotation, and the functions of these four genes were verified through virus-induced gene silencing (VIGS). Compared to the negative controls, plants in which any of these four genes were silenced showed significant reductions in fiber strength. In addition, the plants in which the Gomus.A12G023400 gene was silenced showed a significant reduction in fiber uniformity, whereas the plants in which Gomus.A05G281300 was silenced showed a significant increase in fiber fineness as measured via micronaire. Our results showed that these genes play different roles during fiber development, impacting fiber quality.

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