Glucometer-based biosensor for the determination of ractopamine in animal-derived foods using rolling circle amplification
文献类型： 外文期刊
作者： He, Feng 1 ; Li, Tengfei 2 ; Wang, Haijie 1 ; Du, Pengfei 1 ; Wang, Weiting 1 ; Tan, Tianyu 2 ; Liu, Yaobo 1 ; Wang, Shoujing 1 ; Ma, Yanli 1 ; Wang, Yuanshang 1 ; Hu, Peng 1 ; Abd El-Aty, A. M. 5 ;
作者机构： 1.Shandong Acad Agr Sci, Minist Agr, Key Lab Novel Food Resources Proc, Inst Food & Nutr Sci & Technol,Shandong Prov Key L, Jinan 250100, Peoples R China
2.Hebei Univ Engn, Sch Life Sci & Food Engn, Handan 056038, Peoples R China
3.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China
4.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
5.Qilu Univ Technol, Shandong Acad Sci, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Peoples R China
6.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
7.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkiye
关键词： Ractopamine; Rolling circle amplification; Glucometer; Gold nanoparticles; Portable sensing
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.7； 五年影响因子：5.4 ）
ISSN： 0026-3672
年卷期： 2023 年 190 卷 4 期
页码：
收录情况： SCI
摘要： Screening for persistent organic pollutants (POPs) in food is a complex and challenging process, as POPs can be present in very low levels and can be difficult to detect. Herein, we developed an ultrasensitive biosensor based on a rolling circle amplification (RCA) platform using a glucometer to determine POP. The biosensor was constructed using gold nanoparticle probes modified with antibodies and dozens of primers, magnetic microparticle probes conjugated with haptens, and targets. After competition, RCA reactions are triggered, numerous RCA products hybridize with the ssDNA-invertase, and the target is successfully transformed into glucose. Using ractopamine as a model analyte, this strategy obtained a linear detection range of 0.038-5.00 ng mL(-1) and a detection limit of 0.0158 ng mL(-1), which was preliminarily verified by screening in real samples. Compared with conventional immunoassays, this biosensor utilizes the high efficiency of RCA and the portable properties of a glucometer, which effectively improves the sensitivity and simplifies the procedures using magnetic separation technology. Moreover, it has been successfully applied to ractopamine determination in animal-derived foods, revealing its potential as a promising tool for POP screening.
- 相关文献
作者其他论文 更多>>
-
Co-expression of metabolites and sensory attributes through weighted correlation network analysis to explore flavor-contributing factors in various Pyrus spp. Cultivars
作者：Zhang, Wenjun;Du, Hongxia;Chen, Zilei;Mao, Jiangsheng;Zhu, Chao;Yan, Mengmeng;Qin, Hongwei;Bai, Bo;Hao, Qian;Zhang, Lulu;Abd El-Aty, A. M.;Abd El-Aty, A. M.
关键词：Pyrus spp.; Potential flavor factors; Metabolomics; Sensory attributes; Weighted correlation network analysis
-
Facile fabricate sandwich-structured molecularly imprinted dopamine polymer for simultaneously specific capture of Low-density lipoprotein and eliminate "bad cholesterol"
作者：Cao, Jianfang;Liu, Yaobo;Wang, Weiting;Du, Pengfei;Ma, Yanli;Liu, Gang;Wang, Yuanshang
关键词：Sandwich-type; Molecular imprinting; Imprinted cavity; Simultaneously specific capture; Low-density lipoprotein
-
Aptamer and sodium alginate decorated graphene oxide composite material with ion responsiveness for Low-density lipoprotein trapping
作者：Cao, Jianfang;Liu, Yaobo;Wang, Weiting;Du, Pengfei;Ma, Yanli;Wang, Yuanshang;Jin, Linshi
关键词：Graphene oxide; Sodium alginate; Stimuli-responsive; Low-density lipoprotein; Separation
-
Fabrication of carvacrol loaded cellulose acetate phthalate/shellac composite film and its application to mackerel fillets preservation
作者：Dong, Qingfeng;Dai, Yaqi;Li, Li;Wang, Weiting;Ma, Yanli
关键词：Cellulose acetate phthalate; Shellac; Packaging
-
Chitosan-Enhanced pH-Sensitive Anthocyanin Indicator Film for the Accurate Monitoring of Mutton Freshness
作者：Ma, Yanli;Wen, Lei;Liu, Yaobo;Du, Pengfei;Hu, Peng;Cao, Jianfang;Wang, Weiting;Wen, Lei
关键词：anthocyanin; chitosan; pH response sensitivity; indicator
-
An Innovative and Efficient Fluorescent Detection Technique for Salmonella in Animal-Derived Foods Using the CRISPR/Cas12a-HCR System Combined with PCR/RAA
作者：Wang, Yuanshang;Shao, Yanchun;Wang, Xiaohong;Wang, Yuanshang;Shao, Yanchun;Wang, Xiaohong;Wang, Yuanshang;Du, Pengfei;Wang, Weiting;Liu, Yaobo;Ma, Yanli;Hu, Peng;Cao, Jianfang;Abd El-Aty, A. M.;Abd El-Aty, A. M.
关键词：CRISPR/Cas12a-HCR; PCR/RAA; specific target; Salmonella; fluorescence rapid detection; animal-derived foods
-
Research progress on natural preservatives of meat and meat products: classifications, mechanisms and applications
作者：Ma, Yanli;Wen, Lei;Liu, Yaobo;Wang, Weiting;Du, Pengfei;Hu, Peng;Cao, Jianfang;Ma, Yanli;Wen, Lei;He, Hongjun
关键词：natural bacteriostat; chilled meat; food preservation; antibacterial mechanism; controlled release
