An Innovative and Efficient Fluorescent Detection Technique for Salmonella in Animal-Derived Foods Using the CRISPR/Cas12a-HCR System Combined with PCR/RAA
文献类型： 外文期刊
作者： Wang, Yuanshang 1 ; Du, Pengfei 3 ; Shao, Yanchun 1 ; Wang, Weiting 3 ; Liu, Yaobo 3 ; Ma, Yanli 3 ; Hu, Peng 3 ; Cao, Jianfang 3 ; Wang, Xiaohong 1 ; Abd El-Aty, A. M. 4 ;
作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China
3.Shandong Acad Agr Sci, Inst Food & Nutr Sci & Technol, Shandong Prov Key Lab Agroprod Proc Technol, Key Lab Novel Food Resources Proc,Minist Agr, Jinan 250100, Peoples R China
4.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
5.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkiye
关键词： CRISPR/Cas12a-HCR; PCR/RAA; specific target; Salmonella; fluorescence rapid detection; animal-derived foods
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 15 期
页码：
收录情况： SCI
摘要： Here, we present a method for Salmonella detection using clustered regularly interspaced short palindromic repeats associated with the CRISPR-associated protein 12a-hybridization chain reaction (CRISPR/Cas12a-HCR) system combined with polymerase chain reaction/recombinase-assisted amplification (PCR/RAA) technology. The approach relies on the Salmonella invA gene as a biorecognition element and its amplification through PCR and RAA. In the presence of the target gene, Cas12a, guided by crRNA, recognizes and cleaves the amplification product, initiating the HCR. Fluorescently labeled single-stranded DNA (ssDNA) H1 and H2 were introduced, and the Salmonella concentration was determined based on the fluorescence intensity from the triggered HCR. Both assays demonstrate high specificity, sensitivity, simplicity, and rapidity. The detection range was 2 x 10(1)-2 x 10(9) CFU/mL, with an LOD of 20 CFU/mL, and the entire process enabled specific and rapid Salmonella detection within 85-105 min. Field-incurred spiked recovery tests were conducted in mutton and beef samples using both assays, demonstrating satisfactory recovery and accuracy in animal-derived foods. By combining CRISPR/Cas12a with hybridization chain reaction technology, this study presents a rapid and sensitive Salmonella detection method that is crucial for identifying pathogenic bacteria and monitoring food safety.
- 相关文献
作者其他论文 更多>>
-
Co-expression of metabolites and sensory attributes through weighted correlation network analysis to explore flavor-contributing factors in various Pyrus spp. Cultivars
作者：Zhang, Wenjun;Du, Hongxia;Chen, Zilei;Mao, Jiangsheng;Zhu, Chao;Yan, Mengmeng;Qin, Hongwei;Bai, Bo;Hao, Qian;Zhang, Lulu;Abd El-Aty, A. M.;Abd El-Aty, A. M.
关键词：Pyrus spp.; Potential flavor factors; Metabolomics; Sensory attributes; Weighted correlation network analysis
-
Facile fabricate sandwich-structured molecularly imprinted dopamine polymer for simultaneously specific capture of Low-density lipoprotein and eliminate "bad cholesterol"
作者：Cao, Jianfang;Liu, Yaobo;Wang, Weiting;Du, Pengfei;Ma, Yanli;Liu, Gang;Wang, Yuanshang
关键词：Sandwich-type; Molecular imprinting; Imprinted cavity; Simultaneously specific capture; Low-density lipoprotein
-
Aptamer and sodium alginate decorated graphene oxide composite material with ion responsiveness for Low-density lipoprotein trapping
作者：Cao, Jianfang;Liu, Yaobo;Wang, Weiting;Du, Pengfei;Ma, Yanli;Wang, Yuanshang;Jin, Linshi
关键词：Graphene oxide; Sodium alginate; Stimuli-responsive; Low-density lipoprotein; Separation
-
Fabrication of carvacrol loaded cellulose acetate phthalate/shellac composite film and its application to mackerel fillets preservation
作者：Dong, Qingfeng;Dai, Yaqi;Li, Li;Wang, Weiting;Ma, Yanli
关键词：Cellulose acetate phthalate; Shellac; Packaging
-
Chitosan-Enhanced pH-Sensitive Anthocyanin Indicator Film for the Accurate Monitoring of Mutton Freshness
作者：Ma, Yanli;Wen, Lei;Liu, Yaobo;Du, Pengfei;Hu, Peng;Cao, Jianfang;Wang, Weiting;Wen, Lei
关键词：anthocyanin; chitosan; pH response sensitivity; indicator
-
Research progress on natural preservatives of meat and meat products: classifications, mechanisms and applications
作者：Ma, Yanli;Wen, Lei;Liu, Yaobo;Wang, Weiting;Du, Pengfei;Hu, Peng;Cao, Jianfang;Ma, Yanli;Wen, Lei;He, Hongjun
关键词：natural bacteriostat; chilled meat; food preservation; antibacterial mechanism; controlled release
-
Exploring the Aroma Fingerprint of Various Chinese Pear Cultivars through Qualitative and Quantitative Analysis of Volatile Compounds Using HS-SPME and GCxGC-TOFMS
作者：Zhang, Wenjun;Yan, Mengmeng;Chen, Zilei;Li, Huidong;Mao, Jiangsheng;Qin, Hongwei;Zhu, Chao;Du, Hongxia;Zhang, Wenjun;Yan, Mengmeng;Chen, Zilei;Li, Huidong;Mao, Jiangsheng;Qin, Hongwei;Zhu, Chao;Du, Hongxia;Zheng, Xinxin;Abd El-Aty, A. M.;Abd El-Aty, A. M.
关键词：Pyrus ussuriensis Maxim; aroma characteristics; aroma profiles; GCxGC-TOFMS; HS-SPME; quality evaluation
