Facile fabricate sandwich-structured molecularly imprinted dopamine polymer for simultaneously specific capture of Low-density lipoprotein and eliminate "bad cholesterol"
文献类型： 外文期刊
作者： Cao, Jianfang 1 ; Liu, Yaobo 1 ; Wang, Weiting 1 ; Du, Pengfei 1 ; Liu, Gang 2 ; Ma, Yanli 1 ; Wang, Yuanshang 3 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Food & Nutr Sci & Technol, Shandong Prov Key Lab Agroprod Proc Technol, Key Lab Novel Food Resources Proc,Minist Agr, Jinan 250100, Peoples R China
2.Shandong Prov Anim Husb Gen Stn, Jinan 250100, Peoples R China
3.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
关键词： Sandwich-type; Molecular imprinting; Imprinted cavity; Simultaneously specific capture; Low-density lipoprotein
期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.1； 五年影响因子：3.7 ）
ISSN： 0021-9673
年卷期： 2024 年 1724 卷
页码：
收录情况： SCI
摘要： A simplified approach for preparation of sandwich type molecularly imprinted polymers (PPDA-MIPs) is proposed for simultaneously identify Low-density lipoprotein (LDL) and dispose "bad cholesterol". Porous polydopamine nanosphere (PPDA) is applied as a matrix for immobilization of LDL, and the imprinted layer is formed by dopamine acting as a functional monomer. Since imprinted cavities exhibit shape memory effects in terms of recognizing selectivity, the PPDA-MIPs exhibit excellent selectivity toward LDL and a substantial binding capacity of 550.3 mu g mg-1. Meanwhile, six adsorption/desorption cycles later, the adsorption efficiency of 83.09 % is still achieved, indicating the adequate stability and reusability of PPDA-MIPs. Additionally, over 80 % of cholesterol is recovered, indicating the completeness of "bad cholesterol" removal in LDL. Lastly, as demonstrated by gel electrophoresis, PPDA-MIPs performed satisfactory behavior for the removal of LDL from the goat serum sample.
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