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Wheat Elongator Subunit 4 Negatively Regulates Freezing Tolerance by Regulating Ethylene Accumulation

文献类型： 外文期刊

作者： Wang, Kai 1 ; Zhai, Mingjuan 2 ; Han, Ran 1 ; Wang, Xiaolu 1 ; Xu, Wenjing 1 ; Zeng, Xiaoxue 1 ; Qi, Guang 1 ; Komatsuda, Takao 1 ; Liu, Cheng 1 ;

作者机构： 1.Shandong Acad Agr Sci, Natl Engn Res Ctr Wheat & Maize, Shandong Technol Innovat Ctr Wheat, Crop Res Inst, Jinan 252100, Peoples R China

2.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词： Elongator complex; freezing stress; histone acetylation; ethylene; Triticum aestivum

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 14 期

页码：

收录情况： SCI

摘要： Freezing stress is a major factor limiting production and geographical distribution of temperate crops. Elongator is a six subunit complex with histone acetyl-transferase activity and is involved in plant development and defense responses in Arabidopsis thaliana. However, it is unknown whether and how an elongator responds to freezing stress in plants. In this study, we found that wheat elongator subunit 4 (TaELP4) negatively regulates freezing tolerance through ethylene signaling. TaELP4 promoter contained cold response elements and was up-regulated in freezing stress. Subcellular localization showed that TaELP4 and AtELP4 localized in the cytoplasm and nucleus. Silencing of TaELP4 in wheat with BSMV-mediated VIGS approach significantly elevated tiller survival rate compared to control under freezing stress, but ectopic expression of TaELP4 in Arabidopsis increased leaf damage and survival rate compared with Col-0. Further results showed that TaELP4 positively regulated ACS2 and ACS6 transcripts, two main limiting enzymes in ethylene biosynthesis. The determination of ethylene content showed that TaELP4 overexpression resulted in more ethylene accumulated than Col-0 under freezing stress. Epigenetic research showed that histone H3K9/14ac levels significantly increased in coding/promoter regions of AtACS2 and AtACS6 in Arabidopsis. RT-qPCR assays showed that the EIN2/EIN3/EIL1-CBFs-COR pathway was regulated by TaELP4 under freezing stress. Taken together, our results suggest that TaELP4 negatively regulated plant responses to freezing stress via heightening histone acetylation levels of ACS2 and ACS6 and increasing their transcription and ethylene accumulation.

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[1]Wheat Elongator Subunit 4 Negatively Regulates Freezing Tolerance by Regulating Ethylene Accumulation. Wang, Kai,Han, Ran,Wang, Xiaolu,Xu, Wenjing,Zeng, Xiaoxue,Qi, Guang,Komatsuda, Takao,Liu, Cheng,Zhai, Mingjuan. 2022

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