Bulked segregant RNA-seq reveals complex resistance expression profile to powdery mildew in wild emmer wheat W762
文献类型： 外文期刊
作者： Qian, Zejun 1 ; Liu, Ruishan 1 ; Liu, Xueqing 2 ; Qie, Yanmin 3 ; Wang, Jiangchun 2 ; Yin, Yan 2 ; Xin, Qingguo 2 ; Yu, Ningning 1 ; Zhang, Jiadong 1 ; Li, Yaoxue 1 ; Li, Jiatong 1 ; Dai, Yintao 1 ; Liu, Cheng 4 ; Jin, Yuli 1 ; Ma, Pengtao 1 ;
作者机构： 1.Yantai Univ, Coll Life Sci, Yantai Key Lab Characterist Agr Bioresource Conser, Yantai, Peoples R China
2.Inst Grain & Oil Crops, Yantai Acad Agr Sci, Yantai, Peoples R China
3.Inst Cereal & Oil Crops, Hebei Acad Agr & Forestry Sci, Hebei Lab Crop Genet & Breeding, Shijiazhuang, Peoples R China
4.Crop Res Inst, Shandong Acad Agr Sci, Jinan, Peoples R China
关键词： durum wheat; powdery mildew; BSR-seq; expression profiling; DEG
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive fungal diseases threatening global wheat production. Exploring powdery mildew resistance (Pm) gene(s) and dissecting the molecular mechanism of the host resistance are critical to effectively and reasonably control this disease. Durum wheat (Triticum turgidum L. var. durumDesf.) is an important gene donor for wheat improvement against powdery mildew. In this study, a resistant durum wheat accession W762 was used to investigate its potential resistance component(s) and profile its expression pattern in responding to Bgt invasion using bulked segregant RNA-Seq (BSR-Seq) and further qRT-PCR verification. Genetic analysis showed that the powdery mildew resistance in W762 did not meet monogenic inheritance and complex genetic model might exist within the population of W762 x Langdon (susceptible durum wheat). After BSR-Seq, 6,196 consistently different single nucleotide polymorphisms (SNPs) were called between resistant and susceptible parents and bulks, and among them, 763 SNPs were assigned to the chromosome arm 7B. Subsequently, 3,653 differentially expressed genes (DEGs) between resistant and susceptible parents and bulks were annotated and analyzed by Gene Ontology (GO), Cluster of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. The potential regulated genes were selected and analyzed their temporal expression patterns following Bgt inoculation. As a result, nine disease-related genes showed distinctive expression profile after Bgt invasion and might serve as potential targets to regulate the resistance against powdery mildew in W762. Our study could lay a foundation for analysis of the molecular mechanism and also provide potential targets for the improvement of durable resistance against powdery mildew.
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