文献类型： 外文期刊

作者： Thirulogachandar, Venkatasubbu ¹ ; Govind, Geetha ¹ ; Hensel, Goetz ¹ ; Kale, Sandip M. ¹ ; Kuhlmann, Markus ¹ ; Eschen-Lippold, Lennart ³ ; Rutten, Twan ¹ ; Koppolu, Ravi ¹ ; Rajaraman, Jeyaraman ¹ ; Palakolanu, Sudhakar Reddy ¹ ; Seiler, Christiane ¹ ; Sakuma, Shun ⁴ ; Jayakodi, Murukarthick ¹ ; Lee, Justin ³ ; Kumlehn, Jochen ¹ ; Komatsuda, Takao ⁴ ; Schnurbusch, Thorsten ¹ ; Sreenivasulu, Nese ¹ ;

作者机构： 1.Leibniz Inst Plant Genet & Crop Plant Res IPK, Corrensstr 3, D-06466 Stadt Seeland, Germany

2.Interdisciplinary Ctr Crop Plant Res IZN, Res Grp Abiot Stress Genom, Hoher Weg 8, D-06120 Halle, Germany

3.Leibniz Inst Plant Biochem IPB, Weinberg 3, D-06120 Halle, Germany

4.Natl Inst Agrobiol Sci NIAS, Plant Genome Res Unit, Tsukuba 3058602, Japan

5.Martin Luther Univ Halle Wittenberg, Inst Agr & Nutr Sci, Fac Nat Sci 3, D-06120 Halle, Germany

6.Heinrich Heine Univ, Inst Plant Genet, D-40225 Dusseldorf, Germany

7.Coll Agr, Dept Crop Physiol, Hassan 573225, Karnataka, India

8.Heinrich Heine Univ, Inst Plant Biochem, D-40225 Dusseldorf, Germany

9.Dept Agroecol, Crop Genet & Biotechnol Sect, Forsogsvej 1, DK-4200 Slagelse, Denmark

10.Martin Luther Univ Halle Wittenberg, Inst Agr & Nutr Sci, Dept Crop Physiol, D-06120 Halle Wittenberg, Germany

11.Int Crops Res Inst Semi Arid Trop, Cell Mol Biol & Trait Engn Cluster, Hyderabad 502324, Telangana, India

12.Julius Kuehn Inst JKI, Inst Resistance Res & Stress Tolerance, D-06484 Quedlinburg, Germany

13.Tottori Univ, Fac Agr, Tottori 6808550, Japan

14.Shandong Acad Agr Sci SAAS, Crop Res Inst, Jinan 250100, Shandong, Peoples R China

15.Int Rice Res Inst IRRI, Grain Qual & Nutr Ctr, DAPO Box 7777, Manila, Philippines

关键词： Anther and pistil development; barley (Hordeum vulgare); CRISPR; gene duplication; HD-ZIP class I transcription factors; inflorescence architecture; nucleotide diversity; spikelet fertility

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2024 年 75 卷 10 期

页码：

收录情况： SCI

摘要： The HD-ZIP class I transcription factor Homeobox 1 (HvHOX1), also known as Vulgare Row-type Spike 1 (VRS1) or Six-rowed Spike 1, regulates lateral spikelet fertility in barley (Hordeum vulgare L.). It was shown that HvHOX1 has a high expression only in lateral spikelets, while its paralog HvHOX2 was found to be expressed in different plant organs. Yet, the mechanistic functions of HvHOX1 and HvHOX2 during spikelet development are still fragmentary. Here, we show that compared with HvHOX1, HvHOX2 is more highly conserved across different barley genotypes and Hordeum species, hinting at a possibly vital but still unclarified biological role. Using bimolecular fluorescence complementation, DNA-binding, and transactivation assays, we validate that HvHOX1 and HvHOX2 are bona fide transcriptional activators that may potentially heterodimerize. Accordingly, both genes exhibit similar spatiotemporal expression patterns during spike development and growth, albeit their mRNA levels differ quantitatively. We show that HvHOX1 delays the lateral spikelet meristem differentiation and affects fertility by aborting the reproductive organs. Interestingly, the ancestral relationship of the two genes inferred from their co-expressed gene networks suggested that HvHOX1 and HvHOX2 might play a similar role during barley spikelet development. However, CRISPR-derived mutants of HvHOX1 and HvHOX2 demonstrated the suppressive role of HvHOX1 on lateral spikelets, while the loss of HvHOX2 does not influence spikelet development. Collectively, our study shows that through the suppression of reproductive organs, lateral spikelet fertility is regulated by HvHOX1, whereas HvHOX2 is dispensable for spikelet development in barley. In barley, the transcription factor HvHOX1 (also known as VRS1) regulates lateral spikelet fertility through the suppression of reproductive organs, but its paralog, HvHOX2, is dispensable for spikelet development.