您好，欢迎访问山东省农业科学院文献资源数据库平台

Single nucleotide polymorphisms, haplotypes and combined genotypes of LAP3 gene in bovine and their association with milk production traits

文献类型： 外文期刊

作者： Zheng, Xue 1 ; Ju, Zhihua 1 ; Wang, Ji 1 ; Li, Qiuling 1 ; Huang, Jinming 1 ; Zhang, Aiwu 2 ; Zhong, Jifeng 1 ; Wang, Changfa 1 ;

作者机构： 1.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan 250100, Shandong, Peoples R China

2.Jilin Agr Univ, Coll Chinese Med Mat, Changchun 130118, Peoples R China

关键词： Bovine; Single nucleotide polymorphism; LAP3 gene; Haplotype; Milk production traits

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2011 年 38 卷 6 期

页码：

收录情况： SCI

摘要： Leucine aminopeptidase 3 (LAP3) is an aminopeptidase which catalyses the removal of N-terminal amino acids and is involved in protein maturation and degradation. In this study, we detected the polymorphisms of LAP3 gene by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods in 916 individuals from three Chinese cattle breeds including Chinese Holstein, Luxi Yellow and Bohai Black. One novel single nucleotide polymorphism (SNP) (g.24564G > A ss196003366) and four previously deposited SNPs in the GenBank database (g.24794T > G, g.24803T > C, g.24846T > C, g.25415T > C) were detected. Three of the SNPs (g.24794T > G, g.24803T > C, g.24846T > C) were firstly found to be linked completely and regarded as a SNP g.24794M > N by PCR-SSCP and DNA sequencing in the tested breeds. The allelic frequencies and genetic indices of the SNPs were different in three Chinese cattle populations. The SNPs and their genetic effects on milk production traits in Chinese Holsteins were evaluated. Least squares analysis showed that cows with genotype MM had higher fat percentage and protein percentage than genotype NN (P < 0.05); and the cows with g.25415T > C-CC genotype had higher protein rate than ones with TT genotype (P < 0.05). In addition, eight haplotypes and 23 combined genotypes were identified based on the nine genotypes and the association between combined genotypes and milk production traits were analyzed. Statistic results showed that the cows with genotype combination MAT/MGC have higher protein and fat rate and lower SCS. Our finding demonstrated that the LAP3 gene possibly contributed to conducting association analysis and can be used as molecular marker in milk production traits and other performance for animal breeding.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台