Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility
文献类型： 外文期刊
作者： Yang, Chunhong 1 ; Xiao, Yao 1 ; Wang, Xiuge 1 ; Wei, Xiaochao 1 ; Wang, Jinpeng 1 ; Gao, Yaping 1 ; Jiang, Qiang 1 ; Ju, Zhihua 1 ; Zhang, Yaran 1 ; Liu, Wenhao 1 ; Huang, Ning 1 ; Li, Yanqin 1 ; Gao, Yundong 1 ; Wang, Lingling 1 ; Huang, Jinming 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Key Lab Livestock & Poultry Multi MARA, Jinan, Peoples R China
2.Shandong Key Lab Anim Dis Control & Breeding, Jinan, Peoples R China
3.Shandong Normal Univ, Coll Life Sci, Jinan, Peoples R China
4.202 North Ind Rd, Jinan 250000, Shandong, Peoples R China
关键词： Alternative splicing; dairy cattle; epigenetic regulation; PBRM1; spermatogenesis
期刊名称：EPIGENETICS （ 影响因子：3.7； 五年影响因子：4.6 ）
ISSN： 1559-2294
年卷期： 2023 年 18 卷 1 期
页码：
收录情况： SCI
摘要： DNA methylation and gene alternative splicing drive spermatogenesis. In screening DNA methylation markers and transcripts related to sperm motility, semen from three pairs of full-sibling Holstein bulls with high and low motility was subjected to reduced representation bisulphite sequencing. A total of 948 DMRs were found in 874 genes (gDMRs). Approximately 89% of gDMR-related genes harboured alternative splicing events, including SMAD2, KIF17, and PBRM1. One DMR in exon 29 of PBRM1 with the highest 5mC ratio was found, and hypermethylation in this region was related to bull sperm motility. Furthermore, alternative splicing events at exon 29 of PBRM1 were found in bull testis, including PBRM1-complete, PBRM1-SV1 (exon 28 deletion), and PBRM1-SV2 (exons 28-29 deletion). PBRM1-SV2 exhibited significantly higher expression in adult bull testes than in newborn bull testes. In addition, PBRM1 was localized to the redundant nuclear membrane of bull sperm, which might be related to sperm motility caused by sperm tail breakage. Therefore, the hypermethylation of exon 29 may be associated with the production of PBRM1-SV2 in spermatogenesis. These findings indicated that DNA methylation alteration at specific loci could regulate gene splicing and expression and synergistically alter sperm structure and motility.
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