您好，欢迎访问山东省农业科学院文献资源数据库平台

Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome

文献类型： 外文期刊

作者： Zhang, Jun 1 ; Hong, Yan 2 ;

作者机构： 1.Shandong Acad Agr Sci, Cotton Res Ctr, Jinan, Shandong, Peoples R China

2.Nanyang Technol Univ, Sch Biol Sci, Singapore, Singapore

关键词： Transgenic cotton; Transgene/plant genome junctions; Transgenic structure; Transgene repeats; Vector backbone; Conventional PCR; Genomic Southern blot hybridization; Inverse PCR

期刊名称：TRANSGENIC COTTON, 2 EDITION: Methods and Protocols

ISSN： 1064-3745

年卷期： 2019 年 1902 卷

页码：

收录情况： SCI

摘要： In this chapter, we present detailed experimental procedures for investigating integration patterns of transgenes in cotton genome. We use conventional PCR and genomic Southern blot hybridization to characterize integration of T-DNA components and vector backbone fragments. For multiple-copy insertions into the same site (complex loci), transgene/transgene junctions (including canonical and truncated T-DNA and transgene involved vector backbone sequences) are characterized by PCR and sequencing. Inverse PCR and sequencing are used to characterize transgene/cotton genome junctions. Distribution of T-DNA insertion in cotton genome is evaluated by analysis of transgene flanking sequences. The pre-insertion sites can also be cloned and sequenced (based on the flanking sequences) for survey of genomic structure changes brought by transgene integration by comparing a pre-insertion site with corresponding transgene/plant junctions.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台