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Croton tiglium essential oil compounds have anti-proliferative and pro-apoptotic effects in A549 lung cancer cell lines

文献类型： 外文期刊

作者： Niu, Qing-lin 1 ; Sun, Hui 2 ; Liu, Chao 2 ; Li, Juan 3 ; Liang, Chang-xu 4 ; Zhang, Rui-rui 2 ; Ge, Fu-rong 1 ; Liu, Wei 1 ;

作者机构： 1.Shandong Inst Pomol, Shandong Prov Key Lab Fruit Tree Biotechnol Breed, Tai An, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Key Lab Agroprod Proc Technol Shandong Prov, Key Lab Novel Food Resources Proc, Minist Agr & Rural Affairs,Inst Agrofood Sci & Te, Jinan, Shandong, Peoples R China

3.Taian Tradit Chinese Med Hosp, Tai An, Shandong, Peoples R China

4.Univ Jinan, Sch Water Conservancy & Environm, Jinan, Shandong, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2020 年 15 卷 5 期

页码：

收录情况： SCI

摘要： As a traditional Chinese medicine, Croton tiglium has the characteristics of laxative, analgesic, antibacterial and swelling. This study aimed to analyze the chemical composition of C. tiglium essential oil (CTEO) extracted from the seeds of C. tiglium and its cytotoxicity and antitumor effect in vitro. Supercritical CO2 fluid extraction technology was used to extract CTEO and the chemical constituents of the essential oil were identified by comparing the retention indices and mass spectra data taken from the NIST library with those calculated based on the C7-C40 n-alkanes standard. In vitro cytotoxicity of the CTEO was assessed against cancer cell lines (A549) and the human normal bronchial epithelial cells (HBE) using the CCK-8 assay. Proliferation was detected by colony formation experiments. Wound scratch and cell invasion assays were used to detect cell migration and invasion. Levels of apoptotic markers, signaling molecules, and cell cycle regulators expression were characterized by Western blot analysis. As the results, twenty-eight compounds representing 92.39% of the total oil were identified in CTEO. The CTEO has significant antitumor activity on A549 cancer cells (IC50 48.38 mu g/mL). In vitro antitumor experiments showed that CTEO treatment significantly inhibited the proliferation and migration of A549 cells, disrupted the cell cycle process, and reduced the expression levels of cyclin A, cyclin B and CDK1. CTEO can also reduce mitochondrial membrane potential, activate caspase-dependent apoptosis pathway, and finally induce apoptosis. CTEO may become an effective anti-cancer drug and will be further developed for cancer treatment.

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