Newcastle disease virus forms inclusion bodies with features of liquid-liquid phase separation
文献类型： 外文期刊
作者： Wang, Chongyang 1 ; Duan, Liuyuan 1 ; Wang, Ting 1 ; Wang, Wenbin 2 ; Han, Yu 1 ; Hu, Ruochen 1 ; Hou, Qili 1 ; Liu, Haijin 1 ; Wang, Juan 1 ; Wang, Xinglong 1 ; Xiao, Sa 1 ; Dang, Ruyi 1 ; Wang, Junru 3 ; Zhang, Gaiping 1 ; Yang, Zengqi 1 ;
作者机构： 1.Northwest A&F Univ, Coll Vet Med, Yangling, Peoples R China
2.Shandong Acad Agr Sci, Poultry Inst, Jinan, Peoples R China
3.Northwest A&F Univ, Coll Chem & Pharm, Yangling, Peoples R China
4.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Zhengzhou, Peoples R China
关键词： Newcastle disease virus; Inclusion bodies; Liquid-liquid phase separation; Nucleoprotein; Phosphoprotein
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）
ISSN： 0378-1135
年卷期： 2023 年 284 卷
页码：
收录情况： SCI
摘要： Formation of inclusion bodies (IBs) is a hallmark of infections with negative-strand RNA viruses. Although the Newcastle disease virus (NDV) IBs had been observed in the 1950s, the characteristics of NDV IBs remained largely unknown. Here, we show that NDV infection triggers the formation of IBs that contain newly synthesized viral RNA. The structures of NDV IBs, observed by electron microscopy, were not membrane-bound. Fluorescence recovery after photobleaching a region of NDV IBs occurred rapidly, and IBs were dissolved by 1,6-hexanediol treatment, demonstrating they exhibited properties consistent with liquid-liquid phase separation (LLPS). We find the nucleoprotein (NP) and phosphoprotein (P) are sufficient to generate IB-like puncta, with the N arm domain and N core region of NP and the C terminus of P playing important roles in this process. In summary, our findings suggest that NDV forms IBs containing viral RNA, and provide insights into the formation of NDV IBs.
- 相关文献
作者其他论文 更多>>
-
The W195 Residue of the Newcastle Disease Virus V Protein Is Critical for Multiple Aspects of Viral Self-Regulation through Interactions between V and Nucleoproteins
作者：Wei, Qiaolin;Meng, Fanxing;Wang, Ying;Wei, Ning;Tian, Jianxia;Li, Hanlue;Hao, Qiqi;Zhou, Zijie;Liu, Haijin;Yang, Zengqi;Xiao, Sa;Wang, Wenbin
关键词：Newcastle disease virus; V protein; minigenome; inclusion body formation
-
Parcel-Based Sugarcane Mapping Using Smoothed Sentinel-1 Time Series Data
作者：Li, Hongzhong;Wang, Zhengxin;Sun, Luyi;Zhao, Longlong;Li, Xiaoli;Han, Yu;Chen, Jinsong;Wang, Zhengxin;Zhao, Yelong;Liang, Shouzhen
关键词：Sentinel-1A; time series smoothing; sugarcane mapping; parcel-based classification
-
On-site detection and differentiation of African swine fever virus variants using an orthogonal CRISPR-Cas12b/Cas13a-based assay
作者：Wang, Zhe;Wang, Aiping;Zhang, Gaiping;Wang, Zhe;Wang, Aiping;Zhang, Gaiping;Wang, Zhe;Wang, Aiping;Zhang, Gaiping;Wang, Yu;Zhang, Ying;Qin, Guosong;Zhao, Jianguo;Wang, Yu;Zhao, Jianguo;Zhang, Ying;Qin, Guosong;Zhao, Jianguo;Zhang, Ying;Qin, Guosong;Zhao, Jianguo;Sun, Wenbo;Wang, Yanfang;Zhang, Gaiping
关键词：
-
9-Butyl-Harmol Exerts Antiviral Activity against Newcastle Disease Virus through Targeting GSK-3 beta and HSP90 beta
作者：Wang, Chongyang;Wang, Ting;Hu, Ruochen;Duan, Liuyuan;Hou, Qili;Han, Yu;Liu, Haijin;Wang, Xinglong;Xiao, Sa;Yang, Zengqi;Wang, Chongyang;Dai, Jiangkun;Wang, Junru;Wang, Wenbin;Ren, Shanhui;Li, Na
关键词：Newcastle disease virus; beta-carbolines; antiviral mechanism; GSK-3 beta; HSP90 beta
-
The brain-specific upregulation of CARD11 in response to avian brain- neurotropic virus infection serves as a potential biomarker
作者：Wang, Wenbin;Liu, Xuelan;Yan, Peipei;Fu, Chunyan;Zhang, Yajie;Xiao, Sa;Yang, Zengqi
关键词：CARD11; brain-specific upregulation; avian brain-neurotropic virus; potential biomarker
-
Effects of in ovo feeding of t10,c12-conjugated linoleic acid on hepatic lipid metabolism and subcutaneous adipose tissue deposition in newly hatched broiler chicks
作者：Xu, Wentao;Wang, Wenbin;Li, Xia;Yan, Peipei;Shi, Tianhong;Fu, Chunyan;Liu, Xuelan;Xu, Wentao;Song, Zhigang;Fu, Chunyan;Wang, Wenbin;Li, Xia;Yan, Peipei;Shi, Tianhong;Liu, Xuelan;Li, Xia;Yan, Peipei;Shi, Tianhong;Fu, Chunyan;Liu, Xuelan
关键词：t10,c12-conjugated linoleic acid; in ovo feeding; liver; lipid metabolism; newly hatched chicks
-
Capability of Phenology-Based Sentinel-2 Composites for Rubber Plantation Mapping in a Large Area with Complex Vegetation Landscapes
作者：Li, Hongzhong;Zhao, Longlong;Sun, Luyi;Li, Xiaoli;Han, Yu;Chen, Jinsong;Wang, Jin;Liang, Shouzhen
关键词：rubber plantations; Sentinel-2; feature importance; phenology; image compositing
