您好，欢迎访问山东省农业科学院文献资源数据库平台

Partial purification and characterization of polyphenol oxidase and peroxidase from chestnut kernel

文献类型： 外文期刊

作者： Gong, Zhiqing 1 ; Li, Dajing 2 ; Liu, Chunquan 2 ; Cheng, Anwei 1 ; Wang, Wenliang 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Agro Food Sci & Technol, Jinan, Shandong, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Farm Prod Proc, Nanjing, Jiangsu, Peoples R China

关键词： Chestnut;Polyphenol oxidase;Peroxidase;Purification;Characterization

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：4.952； 五年影响因子：5.383 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Presently there is very little information about the properties of polyphenol oxidase (PPO) and peroxidase (POD) from chestnut, which are both related with chestnut browning. The chestnut PPO and POD were partially purified by acetone extraction, 30-80 g/100 mL ammonium sulfate fractionation, DE-52 anion-exchange column. PPO and POD activity increased 4.6-fold and 9.52-fold, its yields were 2.03 U/100 U and 4.21 U/100 U, and its specific activity was 1375 and 16,500 U mg(-1) protein after DE-52. SDS-PAGE results indicated that the molecular mass of PPO and POD was approximately 32.5 and 30.3 kDa, respectively. Its optima pH value for catalyzing catechol and 2-hydroxy phenol was both 7.0. The optimum temperature of PPO and POD for catalyzing catechol and 2-hydroxy phenol was found to be 40 and 50 degrees C, respectively. PPO showed the highest affinity to catechol among all our selected substrates. The K-m and V-max of PPO for substrate catechol were 92 mmol/L and 1.53 Delta OD/min. The K-m and V-max of POD for substrate 2-hydroxy phenol were 49 mmol/L and 0.2373 Delta OD/min. The investigation on the properties of PPO and POD is greatly important for minimizing the losses caused by fruit browning during chestnut processing. (C) 2014 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat. He, Xue,Fang, Jingjing,Li, Jingjuan,Qu, Baoyuan,Ren, Yongzhe,Ma, Wenying,Zhao, Xueqiang,Li, Bin,Wang, Daowen,Li, Zhensheng,Tong, Yiping,Fang, Jingjing,Li, Jingjuan,Ren, Yongzhe. 2014

[2]Deep Roots are Pivotal for Regulating Post-Anthesis Leaf Senescence in Wheat (Triticum aestivum L.). Kong, L.,Si, J.,Sun, M.,Feng, B.,Zhang, B.,Li, S.,Wang, Z.,Wang, F.,Wang, F.. 2013

[3]Water-soluble phenolic compounds in the coat control germination and peroxidase reactivation in Triticum aestivum seeds. Kong, Lingan,Wang, Fahong,Si, Jisheng,Feng, Bo,Li, Shengdong.

[4]Purification and characterization of a cis-epoxysuccinic acid hydrolase from Bordetella sp strain 1-3. Li, Xia,Lu, Hongbo,Ma, Xiaohang,Kai, Lei,Guo, Kangping,Zhao, Yuhua,Li, Xia,Xu, Tongcheng. 2010

[5]An acid-tolerant lectin coupled with high Hg2+ potentiated hemagglutination enhancing property purified from Amanita hemibapha var. ochracea. Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Wang, Bo,Gong, Zhiyuan,Ng, Tzi Bun. 2014

[6]Presence of prophenoloxidase in the humoral fluid of amphioxus Branchiostoma belcheri tsingtauense. Pang, QX,Zhang, SC,Wang, CF,Shi, XD,Sun, YN.

[7]Purification and characterization of a ribonuclease with antiproliferative activity from the mystical wild mushroom Tuber indicum. Feng, ShanShan,Wang, HeXiang,Xiao, Chen,Feng, ShanShan,Wang, HeXiang,Gong, ZhiYuan,Ng, TziBun.

[8]Single-step chromatography for simultaneous purification of C-phycocyanin and allophycocyanin with high purity and recovery from Spirulina (Arthrospira) platensis. Yan, Shi-Gan,Su, Hai-Nan,Zhang, Xi-Ying,Chen, Xiu-Lan,Zhou, Bai-Cheng,Zhang, Yu-Zhong,Zhu, Li-Ping,Yan, Shi-Gan.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台