Novel electrochemiluminescence sensing platform for ultrasensitive detection of malathion residue in tea based on SiO2NSs 2 NSs doped Luminol/ AgNPs as a signal amplification strategy
文献类型： 外文期刊
作者： Li, Peisen 1 ; Sun, Jiashuai 1 ; Wang, Haifang 4 ; Huang, Jingcheng 1 ; Geng, Lingjun 1 ; Dong, Haowei 1 ; Li, Donghan 1 ; Li, Chengqiang 1 ; Fang, Mingxuan 1 ; Zhang, Xin 1 ; Song, Lubin 5 ; Guo, Yemin 1 ; Sun, Xia 1 ;
作者机构： 1.Shandong Univ Technol, Coll Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Shandong Prov Engn Res Ctr Vegetable Safety & Qual, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
4.Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100700, Peoples R China
5.Shandong Acad Agr Sci, Tea Res Inst, Jinan 250100, Shandong, Peoples R China
关键词： Electrochemiluminescence; Silver nanoparticles; Aptasensor; Malathion; Tea
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：12.2； 五年影响因子：11.9 ）
ISSN： 0304-3894
年卷期： 2024 年 477 卷
页码：
收录情况： SCI
摘要： To address the potential hazards of organophosphorus pesticides (OPs) residues in tea, an electrochemiluminescence (ECL) aptasensor based on functionalized nanomaterials was constructed in this work. Firstly, gold nanoparticles (AuNPs) were attached on the surface of multi-walled carbon nanotubes (MWCNTs) by the constant potential electrodeposition to form a compound, and it was utilized to provide excellent immobilization sites for complementary DNA (cDNA). Subsequently, composite nanomaterials were synthesized by a one-pot method with aminated Luminol/silver nanoparticles@silica nanospheres (NH2-Luminol/Ag@SiO2NSs). 2-Luminol/Ag@SiO 2 NSs). Finally, NH2-Luminol/Ag@SiO2NSs 2-Luminol/Ag@SiO 2 NSs was combined with a malathion aptamer (Apt) to obtain signal probes (SPs) for the construction of an aptasensor. The aptasensor had a wide linear range (1x10-- 3-1x10 3 ng/mL) and a low limit of detection (LOD) (0.3x10-- 3 ng/mL). It had the virtues of high sensitivity, wonderful stability and excellent specificity, which could be used for the detection of malathion residue in tea. The work provides a proven way for the construction of a rapid and ultrasensitive aptasensor with low-cost.
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