Immediate, sensitive and specific time-resolved fluorescent immunoassay strips based on immune competition for the detection of procymidone in vegetables
文献类型： 外文期刊
作者： Li, Gaozhen 1 ; Guan, Fukai 1 ; Zhao, Shancang 4 ; Xu, Huihui 1 ; Sun, Jiashuai 1 ; Huang, Jingcheng 1 ; Yang, Qingqing 1 ; Guo, Yanyin 1 ; Sun, Xia 1 ; Guo, Yemin 1 ;
作者机构： 1.Shandong Univ Technol, Sch Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Shandong Prov Engn Res Ctr Vegetable Safety & Qual, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
4.Shandong Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, 23788 Gongye North Rd, Jinan 25100, Shandong, Peoples R China
关键词： Procymidone; Vegetables; Time -resolved fluorescent immunoassay strip; Ultrahigh -performance liquid chromatography; tandem mass spectrometry
期刊名称：FOOD CONTROL （ 影响因子：6.0； 五年影响因子：5.8 ）
ISSN： 0956-7135
年卷期： 2023 年 147 卷
页码：
收录情况： SCI
摘要： As an organochlorine pesticide, procymidone has become an increasing serious problem in vegetables in recent years. In order to solve a series of problems such as low sensitivity of the immunological methods, a timeresolved fluorescent immunoassay strip (TRFIAS) was developed with low limit of detection (LOD). Based on the principle of antigen-antibody immune competition, TRFIAS could detect procymidone in vegetables. After the targets being added, the samples were incubated at 37 degrees C for 10 min. TRFIAS was observed under a UV lamp of 365 nm. The results were processed by ImageJ software to complete the qualitative and quantitative analysis of TRFIAS. Under the optimal working conditions, a linear range of TRFIAS was 5-250 ng/mL with a LOD of 0.33 ng/mL and a visual limit of detection (VDL) of 50 ng/mL. And the TRFIAS had good specificity, precision and accuracy. Comparing the results of TRFIAS with those of ultrahigh-performance liquid chromatographytandem mass spectrometry (UPLC-MS/MS), the two assays were in good agreement. Therefore, TRFIAS has the advantages of high sensitivity, high specificity, simple operation and relatively short assay time. Meanwhile, it shows absolutely great advantages in both quantitative and qualitative analysis.
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