Competitive Electrochemical Immunosensor for Detection of Capsaicinoids in Edible Vegetable Oil Based on Computer Aided Molecular Modeling
文献类型： 外文期刊
作者： Sun, Xia 1 ; Zhao, Qingxue 1 ; Zha, Chuanyun 1 ; Zhang, Jiali 1 ; Zhou, Zhongrui 1 ; Dong, Haowei 1 ; Yang, Qingqing 1 ; Guo, Yemin 1 ; Zhao, Shancang 1 ;
作者机构： 1.Shandong Univ Technol, Sch Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Shandong Prov Engn Res Ctr Vegetable Safety & Qua, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
4.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250000, Shandong, Peoples R China
关键词： Capsaicinoids; Molecular Modeling; Antibody Recognition; Electrochemical Immunosensor; Edible Vegetable Oil
期刊名称：JOURNAL OF THE ELECTROCHEMICAL SOCIETY （ 影响因子：4.386； 五年影响因子：4.371 ）
ISSN： 0013-4651
年卷期： 2022 年 169 卷 5 期
页码：
收录情况： SCI
摘要： The adulteration of edible vegetable oil is a serious threat to the health of consumers. Previous studies have shown that capsaicinoids could be used as a marker of adulteration to identify vegetable oils. The key sites of antibody and capsaicinoids were analyzed by computer aided molecular modeling (CAMM), which provided a basis for the selection of modified material and the implanting of antigen and antibody on the electrode in the subsequent construction of the electrochemical immunosensor. Simultaneously, it was determined that the different forces between capsaicinoids and antibody were the important reason for the differences in sensitivity of capsaicinoids. On this basis, combined with the good conductivity and excellent redox characteristic of Fe(3)O(4)NPs@CeO2, a competitive immunosensor was constructed. Under the optimal conditions, the proposed immunosensor provided a linear range of 10(-3) to 10(4) ng ml(-1) with a detection limit of 0.33 pg ml(-1). This study may broaden the interaction between capsaicinoids and antibody, encourage the design of novel performance-enhanced antibody, and provide a new method for the detection of capsaicinoids.
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