Multiplex strategy electrochemical platform based on self-assembly dual-site DNA tetrahedral scaffold for one-step detection of diazinon and profenofos
文献类型： 外文期刊
作者： Wang, Guanjie 1 ; Sun, Jiashuai 1 ; Li, Baoxin 1 ; Guan, Fukai 1 ; Huang, Jingcheng 1 ; Dong, Haowei 1 ; Zhang, Jiali 1 ; Han, Jie 1 ; Shen, Zheng 1 ; Xu, Deyan 1 ; Sun, Xia 1 ; Guo, Yemin 1 ; Zhao, Shancang 1 ;
作者机构： 1.Shandong Univ Technol, Sch Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Shandong Prov Engn Res Ctr Vegetable Safety & Qual, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
4.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Shandong, Peoples R China
关键词： Electrochemical aptasensor; DNA tetrahedron scaffold; Thionine; 6-(Ferrocenyl)hexanethiol; Simultaneous detection
期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：9.8； 五年影响因子：9.6 ）
ISSN： 0048-9697
年卷期： 2023 年 868 卷
页码：
收录情况： SCI
摘要： In the work, based on self-assembly dual-site DNA tetrahedral scaffold (DTS), thionine (Thi), and 6-(Ferrocenyl) hexanethiol (Fc6S), a multiplex strategy electrochemical platform was fabricated for the simultaneous detection of profenofos (PFF) and diazinon (DZN). Thi and Fc6S were used to label aptamers for the synthesis of probes respectively. Notably, Thi and Fc6S engendered recognizable DPV peaks at different potentials to achieve simultaneous detection of PFF and DZN. In addition to increasing the conductivity of the electrode, the combination of carboxylic acid functionalized multi-walled carbon nanotubes and ferroferric oxide nanoparticles could also increase its higher specific surface area of the electrode interface to adsorb more DTS. Because of the mechanical rigidity of the DTS, the DTS could keep a complementary chain upright and provide more binding sites for aptamers, the binding efficiency between the complementary chain and 2 binding aptamers could be improved. Comparing the aptasensors performance of single-strand DNA with that of the DTS with complementary strands, the benefits of the DTS were highlighted in this system. Under optimal conditions, the detection limits of PFF and DZN were both 3.33 pg/mL and the detection ranges were both 1.00 x 101-1.00 x 107 pg/mL. Meanwhile, the recoveries of PFF and DZN were 87.15%-117.34% and 91.20%-114.19%, respectively. The aptasensor could
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