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Ten alien chromosome additions of Gossypium hirsutum-Gossypium bickii developed by integrative uses of GISH and species-specific SSR markers

文献类型： 外文期刊

作者： Tang, Dong 1 ; Feng, Shouli 1 ; Li, Sai 1 ; Chen, Yu 1 ; Zhou, Baoliang 1 ;

作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, MOE Hybrid Cotton R&D Engn Res Ctr, Nanjing 210095, Jiangsu, Peoples R China

2.Shandong Acad Agr Sci, Key Lab Cotton Breeding & Cultivat Huang Huai Hai, Minist Agr, Cotton Res Ctr, Jinan 250100, Shandong, Peoples R China

关键词： Genomic in situ hybridization (GISH); Gossypium hirsutum; Monosomic alien addition line (MAAL); Simple sequence repeat (SSR); Wild cotton

期刊名称：MOLECULAR GENETICS AND GENOMICS （ 影响因子：3.291； 五年影响因子：3.257 ）

ISSN： 1617-4615

年卷期： 2018 年 293 卷 4 期

页码：

收录情况： SCI

摘要： Gossypium bickii: (2n = 26, G(1)G(1)), a wild diploid cotton, carries many favourable traits. However, these favourable traits cannot be directly transferred into G. hirsutum (2n = 52, AADD) cultivars due to the differences in genomes. Monosomic alien addition lines (MAALs) are considered an invaluable tool for the introgression of genes of interest from wild relatives into cultivated crops. In this study, the G. hirsutum-G. bickii amphidiploid (2n = 78, AADDG(1)G(1)) was backcrossed with G. hirsutum to develop alien additions containing individual G. bickii chromosomes in a G. hirsutum background. Genomic in situ hybridization was employed to detect the number of alien chromosomes added to the backcross progenies. A total of 183 G. bickii-specific DNA markers were developed to discriminate the identities of the G. bickii chromosomes added to G. hirsutum and assess the alien chromosome transmissibility. Chromosomes 4G(b) and 13G(b) showed the highest transmissibility, while chromosomes 1G(b), 7G(b) and 11G(b) showed the lowest. Ten of the 13 possible G. hirsutum-G. bickii MAALs were isolated and characterized, which will lay the foundation for transferring resistance genes of G. bickii into G. hirsutum, as well as for gene assignment, physical mapping, and selective isolation and mapping of cDNAs for particular G. bickii chromosomes. The strategies of how to use MAALs to develop varieties with the trait of interest from wild species (such as glanded plant-glandless seed) were proposed and discussed.

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[1]Ten alien chromosome additions of Gossypium hirsutum-Gossypium bickii developed by integrative uses of GISH and species-specific SSR markers. Tang, Dong,Feng, Shouli,Li, Sai,Chen, Yu,Zhou, Baoliang,Chen, Yu. 2018

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