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A recombination bin-map identified a major QTL for resistance to Tomato Spotted Wilt Virus in peanut (Arachis hypogaea)

文献类型： 外文期刊

作者： Agarwal, Gaurav 1 ; Clevenger, Josh 4 ; Kale, Sandip M. 3 ; Wang, Hui 1 ; Pandey, Manish K. 3 ; Choudhary, Divya 1 ; Y 1 ;

作者机构： 1.USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA

2.Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA

3.Ctr Excellence Genom & Syst Biol, ICRISAT, Hyderabad, India

4.Ctr Appl Genet Technol, Mars Wrigley Confectionery, Athens, GA USA

5.Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA

6.Peanut Res Inst, Shandong Acad Agr Sci, Qingdao, Shandong, Peoples R China

7.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan, Shandong, Peoples R China

8.USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA

9.BGI Shenzhen, Shenzhen, Guangdong, Peoples R China

10.Leibniz Inst Plant Genet & Crop Plant Res IPK, Gatersleben, Germany

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2019 年 9 卷

页码：

收录情况： SCI

摘要： Toma to spotted wilt virus (TSWV) is a devastating disease to peanut growers in the South-eastern region of the United States. Newly released peanut cultivars in recent years are crucial as they have some levels of resistance to TSWV. One mapping population of recombinant inbred line (RIL) used in this study was derived from peanut lines of SunOleic 97R and NC94022. A whole genome re-sequencing approach was used to sequence these two parents and 140 RILs. A recombination bin-based genetic map was constructed, with 5,816 bins and 20 linkage groups covering a total length of 2004 cM. Using this map, we identified three QTLs which were colocalized on chromosome A01. One QTL had the largest effect of 36.51% to the phenotypic variation and encompassed 89.5 Kb genomic region. This genome region had a cluster of genes, which code for chitinases, strictosidine synthase-like, and NBS-LRR proteins. SNPs linked to this QTL were used to develop Kompetitive allele specific PCR (KASP) markers, and the validated KASP markers showed expected segregation of alleles coming from resistant and susceptible parents within the population. Therefore, this bin-map and QTL associated with TSWV resistance made it possible for functional gene mapping, map-based cloning, and marker-assisted breeding. This study identified the highest number of SNP makers and demonstrated recombination bin-based map for QTL identification in peanut. The chitinase gene clusters and NBS-LRR disease resistance genes in this region suggest the possible involvement in peanut resistance to TSWV.

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