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The apple lipoxygenase MdLOX3 positively regulates zinc tolerance

文献类型： 外文期刊

作者： Chen, Guo-Lin 1 ; Wang, Da-Ru 1 ; Liu, Xin 1 ; Wang, Xun 1 ; Liu, Hao-Feng 1 ; Zhang, Chun-Ling 3 ; Zhang, Zhen-Lu 1 ; Li, Lin-Guang 2 ; You, Chun-Xiang 1 ;

作者机构： 1.Shandong Agr Univ, Coll Hort Sci & Engn, Natl Res Ctr Apple Engn & Technol, Natl Key Lab Wheat Improvement, Tai An 271018, Shandong, Peoples R China

2.Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China

3.Shandong Agr & Engn Univ, Jinan, Peoples R China

关键词： Heavy metal; Apple; Lipoxygenase; MdLOX3; Zinc stress tolerance; Scavenging by activated oxygen

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）

ISSN： 0304-3894

年卷期： 2024 年 461 卷

页码：

收录情况： SCI

摘要： Various abiotic stresses, especially heavy metals near factories around the world, limit plant growth and productivity worldwide. Zinc is a light gray transition metal, and excessive zinc will inactivate enzymes in the soil, weaken the biological function of microorganisms, and enter the food chain through enrichment, thus affecting human health. Lipoxygenase (LOX) can catalyze the production of fatty acid derivatives from phenolic triglycerides in plants and is an important pathway of fatty acid oxidation in plants, which usually begins under unfavorable conditions, especially under biotic and abiotic stresses. Lipoxygenase can be divided into 9-LOX and 13-LOX. MdLOX3 is a homolog of AtLOX3 and has been identified in apples (housefly apples). MdLOX3 has a typical conserved lipoxygenase domain, and promoter analysis shows that it contains multiple stress response elements. In addition, different abiotic stresses and hormonal treatments induced the MdLOX3 response. In order to explore the inherent anti-heavy metal mechanism of MdLOX3, this study verified the properties of MdLOX3 based on genetic analysis and overexpression experiments, including plant taproots length, plant fresh weight, chlorophyll, anthocyanins, MDA, relative electrical conductivity, hydrogen peroxide and superoxide anion, NBT \DAB staining, etc. In the experiment, overexpression of MdLOX3 in apple callus and Arabidopsis effectively enhanced the tolerance to zinc stress by improving the ability to clear ROS. Meanwhile, tomato materials with overexpression of ectopia grew better under excessive zinc ion stress. These results indicated that MdLOX3 had a good tolerance to heavy metal zinc. Homologous mutants are more sensitive to zinc, which proves that MdLOX3 plays an important positive role in zinc stressed apples, which broadens the range of action of LOX3 in different plants.

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