您好，欢迎访问山东省农业科学院文献资源数据库平台

The uS10c-BPG2 module mediates ribosomal RNA processing in chloroplast nucleoids

文献类型： 外文期刊

作者： Sun, Xueping 1 ; Singla-Rastogi, Meenu 4 ; Wang, Jingwen 1 ; Zhao, Chuanzhi 1 ; Wang, Xingjun 1 ; Li, Pengcheng 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Crop Germplasm Resources, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China

2.Shandong Prov Key Lab Crop Genet Improvement, Ecol & Physiol, Jinan 250100, Shandong, Peoples R China

3.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Shandong, Peoples R China

4.Indiana Univ, Dept Biol, Bloomington, IN 47405 USA

期刊名称：NUCLEIC ACIDS RESEARCH （ 影响因子：14.9； 五年影响因子：16.4 ）

ISSN： 0305-1048

年卷期： 2024 年

页码：

收录情况： SCI

摘要： In plant chloroplasts, certain ribosomal proteins (RPs) and ribosome biogenesis factors (RBFs) are present in nucleoids, implying an association between nucleoids and ribosome biogenesis. In Arabidopsis, the YqeH-type GTPase Brassinazole-Insensitive Pale Green2 (BPG2) is a chloroplast nucleoid-associated RBF. Here, we investigated the relationship between nucleoids and BPG2-involved ribosome biogenesis steps by exploring how BPG2 targets ribosomes. Our findings demonstrate that BPG2 interacts with an essential plastid RP, uS10c, in chloroplast nucleoids in a ribosomal RNA (rRNA)-independent manner. We also discovered that uS10c is a haploinsufficient gene, as the heterozygous deletion of this gene leads to variegated shoots and chlorophyll aggregation. uS10c is integrated into 30S ribosomal particles when rRNA is relatively exposed and also exists in polysome fractions. In contrast, BPG2 exclusively associates with 30S ribosomal particles. Notably, the interaction between BPG2 and 30S particles is influenced by the absence of uS10c, resulting in BPG2 diffusing in chloroplasts instead of targeting nucleoids. Further, our results reveal that the loss of BPG2 function and the heterozygous deletion of uS10c impair the processing of 16S and 23S-4.5S rRNAs, reduce plastid protein accumulation, and trigger the plastid signaling response. Together, these findings indicate that the uS10c-BPG2 module mediates ribosome biogenesis in chloroplast nucleoids. Graphical Abstract

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台