您好，欢迎访问山东省农业科学院文献资源数据库平台

Single nucleotide polymorphisms, haplotypes and combined genotypes of lactoferrin gene and their associations with mastitis in Chinese Holstein cattle

文献类型： 外文期刊

作者： Huang, Jinming 1 ; Wang, Hongmei 1 ; Wang, Changfa 1 ; Li, Jianbin 1 ; Li, Qiuling 1 ; Hou, Minghai 1 ; Zhong, Jifeng 1 ;

作者机构： 1.Shandong Acad Agr Sci, Ctr Dairy Cattle Res, Jinan 250100, Shandong, Peoples R China

关键词： SNPs;Combined genotype;Haplotype;Lactoferrin gene;Mastitis;Dairy cattle

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Lactoferrin (Lf) is naturally produced by the mammary gland, having biological functions of antibacterial and anti-inflammatory activities. To investigate whether the Lf gene is associated with mastitis in dairy cattle, a DNA sequencing approach was used to identify single nucleotide polymorphisms (SNPs) in the gene. Three previously reported SNPs in the 5' flanking region and one novel SNP in exon1 of Lf gene were identified. A total of 353 individuals from Holstein cattle populations were genotyped for their SNPs using Created Restriction Site PCR (CRS-PCR) and PCR-RFLP methods. Twenty-two and nineteen combinations of three SNPs (g.3440T > G, g.3879_3880insG, and g.4432T > C) and another three SNPs (g.3429G > A, g.3440T > G, g.3879_3880insG) were observed, respectively. The result of haplotype analysis of four SNPs showed that fourteen different haplotypes were identified. Two major haplotypes (GECB and GECA) occurred with a frequency of 22.5 and 18.5% in the study population, respectively. Statistical analyses revealed no significant association between one single SNP of Lf gene and SCS, whereas significant associations between their combined genotypes of three SNPs, haplotype and SCS. Combined genotype EFCDBB and GGEFDD with the lowest SCS were favorable for the mastitis resistance. They may be used as a possible candidate for marker-assisted selection in dairy cattle breeding program.

  • 相关文献

[1]Two splice variants of the bovine lactoferrin gene identified in Staphylococcus aureus isolated from mastitis in dairy cattle. Huang, J. M.,Wang, Z. Y.,Ju, Z. H.,Wang, C. F.,Li, Q. L.,Sun, T.,Hou, Q. L.,Hang, S. Q.,Hou, M. H.,Zhong, J. F.. 2011

[2]The polymorphisms of kappa-casein gene and their associations with milk production traits and expression analysis in Chinese Holstein cattle. Ju, Zhihua,Huang, Jinming,Li, Qiuling,Wang, Hongmei,Zhong, Jifeng,Wang, Changfa. 2011

[3]Six novel single-nucleotide polymorphisms in SPAG11 gene and their association with sperm quality traits in Chinese Holstein bulls. Liu, Xinyan,Ju, Zhihua,Wang, Lingling,Zhang, Yan,Huang, Jingmin,Li, Qiuling,Li, Jianbin,Zhong, Jifeng,Wang, Changfa,Liu, Xinyan,An, Liguo.

[4]Increased expression of a novel splice variant of the complement component 4 (C4A) gene in mastitis-infected dairy cattle. Yang, Y.,Huang, J. M.,Ju, Z. H.,Li, Q. L.,Zhou, L.,Li, R. L.,Li, J. B.,Zhong, J. F.,Wang, C. F.,Yang, Y.,Shi, F. X.,Yang, Y.. 2012

[5]The exon 29 c.3535A > T in the alpha-2-macroglobulin gene causing aberrant splice variants is associated with mastitis in dairy cattle. Wang, Xiuge,Huang, Jinming,Zhao, Lihong,Wang, Changfa,Ju, Zhihua,Li, Qiuling,Qi, Chao,Zhang, Yan,Zhang, Zebin,Zhang, Wei,Hou, Minghai,Zhong, Jifeng,Wang, Xiuge,Yuan, Jinduo,Zhao, Lihong. 2012

[6]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[7]One SNP in the 3'-UTR of HMGB1 gene affects the binding of target bta-miR-223 and is involved in mastitis in dairy cattle. Li, Liming,Huang, Jinming,Ju, Zhihua,Qi, Chao,Zhang, Yan,Li, Qiuling,Wang, Changfa,Miao, Weiran,Zhong, Jifeng,Hou, Minghai,Zhang, Xiaojian,Hang, Suqin. 2012

[8]Variants and Gene Expression of the TLR2 Gene and Susceptibility to Mastitis in Cattle. Huang, Jinming,Liu, Li,Wang, Hongmei,Zhang, Cuixia,Ju, Zhihua,Wang, Changfa,Zhong, Jifeng.

[9]Molecular cloning, promoter analysis, SNP detection of Clusterin gene and their associations with mastitis in Chinese Holstein cows. Wang, Zeying,Wang, Genlin,Huang, Jingmin,Zhong, Jifeng.

[10]A SNP in the 3 '-UTR of HSF1 in dairy cattle affects binding of target bta-miR-484. Li, Q. L.,Xia, P.,Wang, Y. J.,Wu, Z. Y.,Jia, Y. H.,Chang, S. M.,Zhang, Z. F.,Li, Q. L.,Chu, M. X.. 2015

[11]Novel single nucleotide polymorphisms (SNPs) of the bovine STAT4 gene and their associations with production traits in Chinese Holstein cattle. Zhang, Fenfen,Shi, Fangxiong,Zhang, Fenfen,Huang, Jinming,Li, Qiuling,Ju, Zhihua,Li, Jiangbin,Zhong, Jifeng,Wang, Changfa. 2010

[12]Solexa sequencing of novel and differentially expressed microRNAs in testicular and ovarian tissues in Holstein Cattle. Huang, Jinming,Ju, Zhihua,Li, Qiuling,Hou, Qinlei,Wang, Changfa,Li, Jianbin,Li, Rongling,Wang, Lingling,Sun, Tao,Hang, Suqin,Gao, Yundong,Hou, Minghai,Zhong, Jifeng.

[13]牛SLC11A1基因多态性分析. 吕文发,郭洋,王洪梅,王长法,李秋玲,侯明海,仲跻峰. 2010

[14]优质鸡MEF2A基因的SNPs检测及其与屠体性状的相关研究. 周艳,刘益平,蒋小松,杜华锐,朱庆. 2009

[15]中国荷斯坦牛催乳素基因A8398G多态性与产奶性状相关性分析. 王丽娟,李秋玲,王洪梅,李建斌,王志玉,王长法,侯明海,仲跻峰. 2008

[16]荷斯坦种公牛HSF1和HSBP1基因多态性分析. 王延久,李建斌,王长法,鞠志花,黄金明,李荣岭,仲跻峰,何剑斌,李秋玲. 2011

[17]鸡VLDLR基因多态性与蛋黄性状的关联分析. 曾顶国,周艳,雷秋霞,韩海霞,李福伟,李桂明,盛倩,王存芳,逯岩. 2012

[18]牛催乳素基因及其单核苷酸多态性与产奶性状关系的研究进展. 贾祥捷,李秋玲,王长法,杨桂文,仲跻峰. 2010

[19]奶牛PRL基因CRS-PCR多态性与产奶性状的关联分析. 王丽娟,李秋玲,王长法,王洪梅,李建斌,侯明海,高运东,仲跻峰. 2009

[20]鲁中肉羊LHCGR基因突变与其产羔数关联分析. 王翔宇,田志龙,潘林香,王金文,狄冉,刘秋月,胡文萍,马琳,储明星. 2020

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台