您好，欢迎访问山东省农业科学院文献资源数据库平台

Rapid Detection of mrp, epf, and sly Genes by Loop-Mediated Isothermal Amplification in Streptococcus suis

文献类型： 外文期刊

作者： Li, LuLu 1 ; Zhang, Qing 1 ; Zhao, Xiaonan 1 ; Zhou, Yufeng 2 ; Sun, Jian 2 ; Ren, Jinrui 3 ; Zhou, Dong 4 ; Luo, Yan-Bo; 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Shandong, Peoples R China

2.South China Agr Univ, Coll Vet Med, Natl Vet Microbiol Drug Resistance Risk Assessmen, Guangzhou, Peoples R China

3.Shandong Normal Univ, Sch Life Sci, Jinan, Peoples R China

4.Shandong Minhe Anim Husb Co Ltd, Penglai, Peoples R China

关键词： Streptococcus suis; mrp; epf; sly; loop-mediated isothermal amplification

期刊名称：FOODBORNE PATHOGENS AND DISEASE （ 影响因子：3.171； 五年影响因子：3.235 ）

ISSN： 1535-3141

年卷期： 2021 年 18 卷 4 期

页码：

收录情况： SCI

摘要： Streptococcus suis remains a serious threat to the worldwide swine industry and human health. In this study, rapid assays for the detection of three common virulence-related factors (mrp, epf, and sly) were developed, evaluated, and applied. Loop-mediated isothermal amplification (LAMP) primers were designed using Primer Explorer V5 software. The sensitivity and specificity of the LAMP assays were determined based on sample turbidity. For all three genes, LAMP assays were performed at 62 degrees C with a reaction time of 60 min. The detection limit of conventional polymerase chain reaction (PCR) was 1 ng/mu L, 10 pg/mu L, and 100 fg/mu L for the epf, sly, and mrp genes, respectively. For the LAMP assays, the detection limits were 10 pg/mu L, 10 fg/mu L, and 100 fg/mu L for epf, sly, and mrp, respectively, representing sensitivities 100-1000 times higher than those of the PCR assay. Furthermore, when the LAMP assays were applied to clinical strains, the results were consistent with those of the PCR assay, confirming the LAMP assays as rapid and reliable detection techniques. In conclusion, the LAMP assays described in this study have the potential to become standard methods to detect the virulence factors mrp, epf, and sly. To the best of our knowledge, this is the first study to report the application of LAMP to detect the mrp, epf, and sly genes.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台