文献类型： 外文期刊
作者： Song, Wenlong 2 ; Du, Yunfei 2 ; Yang, Chunxiang 2 ; Li, Li 2 ; Wang, Shoujing 1 ; Liu, Yaobo 1 ; Wang, Weiting 1 ;
作者机构： 1.Shandong Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Novel Food Resources Proc, Inst Agrofood Sci & Technol,Key Lab Agroprod Proc, 202 Gongye North Rd, Jinan 250100, Peoples R China
2.Engn Res Ctr Food Thermal Proc Technol, Shanghai 201306, Peoples R China
3.Shanghai Engn Res Ctr Aquat Prod Proc & Preservat, Shanghai 201306, Peoples R China
关键词： Bilayer active film; Mutton; Essential oil; Release kinetics; Illumina MiSeq sequencing
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：4.952； 五年影响因子：5.383 ）
ISSN： 0023-6438
年卷期： 2020 年 133 卷
页码：
收录情况： SCI
摘要： This study aimed to develop a bilayer active film by using polyvinyl alcohol (PVA)/ethylene-vinyl acetate copolymer (EVA) and antioxidant essential oils. The effect of the bilayer active film on the quality and shelf-life of mutton was investigated. PVA laminated material was considered to improve the barrier capacity, while essential oils incorporated into EVA laminated material conferred antioxidant and antimicrobial activity. The effect of the addition of essential oils on the film by mechanical properties, oxygen permeability, scanning electron microscopy (SEM) image, Fourier transform infrared spectroscopy (FTIR), Differential Scanning Calorimetry (DSC) was investigated. Releasing of active compound into mutton and antioxidant activity was investigated to evaluate the films. The antibacterial effects of bilayer active films were subsequently evaluated based on the antibacterial properties of active film and Total viable counts (TVC) of mutton. Microbial community structure in the mutton of different packaging was analyzed by Illumina MiSeq sequencing. Based on total volatile basic nitrogen (TVB-N), the shelf life of mutton was tested. The results showed that the active compound improved flexibility and ductility of active PVA/EVA films. The results also showed a high release of OEO (21.99 mg/kg) and TTO (17.96 mg/kg) as well as high antioxidant and antimicrobial efficiency of PVA/EVA films with essential oils. High-throughput sequencing has shown that the addition of OEO and TTO can reduce the type of dominant bacteria, reducing Acinetobacter by 15.8% and 15.55%, respectively. The release test of active compounds into the mutton showed that the higher contents of the active compounds released, the more microbial proliferation limited, thereby it effectively retarded the increase of TVB-N, which extended the shelf life of mutton by 5-6 days.
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