文献类型： 外文期刊
作者： Qiu, Jiying 1 ; Chen, Leilei 1 ; Zhu, Qingjun 1 ; Wang, Daijie 2 ; Wang, Wenliang 1 ; Sun, Xin 1 ; Liu, Xiaoyong 1 ; Du, Fangling 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Jinan 250100, Peoples R China
2.Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan 250014, Peoples R China
关键词： Antioxidants; Peanut shell; DPPH radical; HPLC separation; TOF-MS
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2012 年 135 卷 4 期
页码：
收录情况： SCI
摘要： Peanut shell, a byproduct in oil production, is rich in natural antioxidants. Here, a rapid and efficient method using DPPH-HPLC-DAD-TOF/MS was used for the first time to screen antioxidants in peanut shell. The method is based on the hypothesis that upon reaction with 1,1-diphenyl-2-picrylhydrazyl (DPPH), the peak areas of compounds with potential antioxidant activities in the HPLC chromatogram will be significantly reduced or disappeared, and the identity confirmation could be achieved by HPLC-DAD-TOF/MS technique. With this method, three compounds possessing potential antioxidant activities were found abundantly in the methanolic extract of peanut shell. They were identified as 5,7-dihydroxychromone, eriodictyol, and luteolin. The contents of these compounds were 0.59, 0.92, and 2.36 mg/g, respectively, and luteolin possessed the strongest radical scavenging capacity. DPPH-HPLC-DAD-TOF/MS assay facilitated rapid identification and determination of natural antioxidants in peanut shell, which may be helpful for value-added utilization of peanut processing byproducts. (C) 2012 Elsevier Ltd. All rights reserved.
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