您好，欢迎访问山东省农业科学院文献资源数据库平台

Natural selection on TMPRSS6 associated with the blunted erythropoiesis and improved blood viscosity in Tibetan pigs

文献类型： 外文期刊

作者： Kong, Xiaoyan 1 ; Dong, Xinxing 1 ; Yang, Shuli 1 ; Qian, Jinhua 2 ; Yang, Jianfa 1 ; Jiang, Qiang 3 ; Li, Xingrun 4 ; Wang, Bo 5 ; Yan, Dawei 1 ; Lu, Shaoxiong 1 ; Zhu, Li 1 ; Li, Gen 1 ; Li, Minjuan 1 ; Yi, Shengnan 1 ; Deng, Mingyue 1 ; Sun, Liyuan 1 ; Zhou, Xiaoxia 1 ; Mao, Huaming 1 ; Gou, Xiao 1 ;

作者机构： 1.Yunnan Agr Univ, Fac Anim Sci & Technol, Kunming, Yunnan, Peoples R China

2.Yuxi Agr Vocat Tech Coll, Dept Anim Sci, Yuxi, Yunnan, Peoples R China

3.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan, Shandong, Peoples R China

4.Dali Vocat & Tech Coll Agr & Forestry, Dept Anim Sci, Dali, Yunnan, Peoples R China

5.Yunnan Univ Tradit Chinese Med, Res Expt Ctr, Kunming, Yunnan, Peoples R China

关键词： Tibetan pigs; TMPRSS6; HGB; CGTG indel; High-altitude adaptation

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2019 年 233 卷

页码：

收录情况： SCI

摘要： Tibetan pigs, indigenous to Tibetan plateau, are well adapted to hypoxia. So far, there have been not any definitively described genes and functional sites responsible for hypoxia adaptation for the Tibetan pig. The whole genome-wide association studies in human suggested that genetic variations in TMPRSS6 was associated with hemoglobin concentration (HGB) and red cell counts (RBC). Here we conducted resequencing of the nearly entire genomic region (40.1 kb) of the candidate gene TMPRSS6 in 40 domestic pigs and 40 wild boars along continuous altitudes and identified 708 SNPs, in addition to an indel (CGTG/-) in the intron 10. We conduct the CGTG indel in 838 domestic pigs, both the CGTG deletion frequency and the pairwise r(2) linkage disequilibrium showed an increase with elevated altitudes, suggesting that TMPRSS6 has been under Darwinian positive selection. As the conserved core sequence of hypoxia-response elements (HREs), the deletion of CGTG in Tibetan pigs decreased the expression levels of TMPRSS6 mRNA and protein in the liver revealed by real-time quantitative PCR and western blot, respectively. We compared domestic pigs and Tibetan pigs living continuous altitudes, found that the blood-related traits with the increase of altitude, however, the HGB did not increase with the elevation in Tibetan pigs. Genotype association analysis results dissected a genetic effect on reducing HGB by 13.25 g/L in Gongbo'gyamda Tibetan pigs, decreasing mean corpuscular volume (MCV) by 4.79 fl in Diqing Tibetan pigs. In conclusion, the CGTG deletion of TMPRSS6 resulted in lower HGB and smaller MCV, which could reflect a blunting erythropoiesis and improving blood viscosity as well as erythrocyte deformability. It remains to be determined whether a blunting of erythropoiesis for TMPRSS6 or others genetic effects are the physiological adaptations among Tibetan pigs.

  • 相关文献

[1]Natural selection on TMPRSS6 associated with the blunted erythropoiesis and improved blood viscosity in Tibetan pigs. Kong, Xiaoyan,Dong, Xinxing,Yang, Shuli,Yang, Jianfa,Yan, Dawei,Lu, Shaoxiong,Zhu, Li,Li, Gen,Li, Minjuan,Yi, Shengnan,Deng, Mingyue,Sun, Liyuan,Zhou, Xiaoxia,Mao, Huaming,Gou, Xiao,Qian, Jinhua,Jiang, Qiang,Li, Xingrun,Wang, Bo. 2019

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台